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COX-2: an in vivo evidence of its participation in heat stress-induced myocardial preconditioning
- Source :
- Cardiovascular Research, Cardiovascular Research, Oxford University Press (OUP), 2003, 58 (3), pp.582-8
- Publication Year :
- 2003
-
Abstract
- Heat stress (HS) is known to induce delayed protection against myocardial infarction. We have previously shown that inducible nitric oxide synthase (iNOS), was involved in mediating this form of preconditioning. Since iNOS and cyclooxygenase-2 (COX-2) are co-induced in various cell types, the goal of this study was to investigate whether COX-2 could also participate to the HS-induced cardioprotection.A total of 78 male Wistar rats, subjected to either heat stress (42 degrees C for 15 min) or sham anaesthesia were used for this study. Twenty-four hours later, they were treated or not with a selective COX-2 inhibitor, either celecoxib (3 mg kg(-1), i.p.) or NS-398 (5 mg kg(-1), i.p.), 30 min before being subjected to a 30-min occlusion of the left coronary artery followed by a 120-min reperfusion, in vivo. HS resulted in a marked increase in myocardial COX-2 protein expression at 24 h, associated with a significant protection against infarction (46.0+/-1.4% in sham vs. 26.8+/-3.8% in HS group) (Por=0.05). Administration of selective COX-2 inhibitor 24 h after HS, completely abrogated this delayed cardioprotection (46.4+/-3.6 and 48.0+/-2.8%, respectively, in HS+celecoxib and HS+NS-398 groups).This study provides the first evidence of an implication of COX-2 as a mediator of HS-induced cardioprotection. This suggests that prostaglandins are involved in this type of cardioprotective preconditioning.
- Subjects :
- Male
Hot Temperature
Physiology
MESH: Cyclooxygenase 2 Inhibitors
Cyclooxygenase-2
MESH: Sulfonamides
Myocardial Ischemia
Infarction
030204 cardiovascular system & hematology
Pharmacology
0302 clinical medicine
MESH: Animals
Myocardial infarction
Cardioprotection
0303 health sciences
Sulfonamides
biology
[SDV.MHEP.CSC] Life Sciences [q-bio]/Human health and pathology/Cardiology and cardiovascular system
Nitric oxide synthase
Isoenzymes
MESH: Ischemic Preconditioning, Myocardial
Anesthesia
Myocardial Ischaemia
Ischemic Preconditioning, Myocardial
MESH: Isoenzymes
MESH: Myocardial Ischemia
MESH: Cyclooxygenase 2
Cardiology and Cardiovascular Medicine
medicine.drug
Heat Stress
MESH: Myocardium
MESH: Rats
MESH: Prostaglandin-Endoperoxide Synthases
Blotting, Western
Ischemia
MESH: Heat
Preconditioning
03 medical and health sciences
MESH: Cyclooxygenase Inhibitors
Reperfusion therapy
[SDV.MHEP.CSC]Life Sciences [q-bio]/Human health and pathology/Cardiology and cardiovascular system
In vivo
Physiology (medical)
medicine
MESH: Blotting, Western
Animals
Cyclooxygenase Inhibitors
Rats, Wistar
Nitrobenzenes
030304 developmental biology
Cyclooxygenase 2 Inhibitors
business.industry
Myocardium
MESH: Nitrobenzenes
MESH: Rats, Wistar
medicine.disease
MESH: Male
Rats
Celecoxib
Cyclooxygenase 2
Prostaglandin-Endoperoxide Synthases
biology.protein
Pyrazoles
business
MESH: Pyrazoles
Subjects
Details
- ISSN :
- 00086363
- Volume :
- 58
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Cardiovascular research
- Accession number :
- edsair.doi.dedup.....cb30d25bb50b72108c76d9e052f603b6