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Evaluation of three extraction-free SARS-CoV-2 RT-PCR assays: A feasible alternative approach with low technical requirements
- Source :
- Journal of Virological Methods
- Publication Year :
- 2020
-
Abstract
- The worldwide demand for SARS-CoV-2 RT-PCR testing resulted in a shortage of diagnostic kits. RNA extraction step constitutes a major bottleneck to perform diagnostic. The aim of this study was to assess performances of different extraction-free SARS-CoV-2 RT-PCR assays compared to a reference RT-PCR assay. The panel of evaluation consisted of 94 samples: 69 positive and 25 negative for SARS-CoV-2 by reference RT-PCR. Three extraction-free RT-PCR assays were assessed: (i) PrimeDirect® Probe RT-qPCR Mix (Takara), (ii) PrimeScript®RT-PCR (Takara), and (iii) SARS-CoV-2 SANSURE®BIOTECH Novel Coronavirus (Sansure). The overall sensitivity of PrimeDirect, PrimeScript and Sansure assays was 55.1 %, 69.6 % and 69.6 %, respectively. The sensitivity increased among samples with Ct
- Subjects :
- 0301 basic medicine
2019-20 coronavirus outbreak
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
030106 microbiology
Pcr assay
RT-PCR
Economic shortage
Biology
Real-Time Polymerase Chain Reaction
Sensitivity and Specificity
Article
03 medical and health sciences
COVID-19 Testing
Virology
Clinical Laboratory Techniques
Reverse Transcriptase Polymerase Chain Reaction
SARS-CoV-2
Extraction (chemistry)
COVID-19
Viral Load
Molecular biology
030104 developmental biology
Real-time polymerase chain reaction
RNA, Viral
RNA extraction
Reagent Kits, Diagnostic
Extraction-Free
Viral load
Subjects
Details
- ISSN :
- 18790984
- Volume :
- 291
- Database :
- OpenAIRE
- Journal :
- Journal of virological methods
- Accession number :
- edsair.doi.dedup.....cb2cf6043da5e004a62e5ab5459119f7