Back to Search
Start Over
Isolating HeLa cell fractions enriched for clathrin-coated vesicles
- Source :
- Cold Spring Harbor protocols. 2014(11)
- Publication Year :
- 2014
-
Abstract
- HeLa cell lines can be experimentally manipulated using drugs or gene-silencing techniques such as RNA interference. Fractions enriched for clathrin-coated vesicles (CCVs) can be isolated from these cell lines and used to study the effects of these manipulations on the composition of CCVs. This protocol, originally developed in the laboratory of Margaret Robinson (Cambridge, United Kingdom), describes the preparation of a HeLa cell fraction that is enriched for a mixed population of CCVs and is suitable for analysis by mass spectroscopy, western blotting, or electron microscopy.
- Subjects :
- education.field_of_study
biology
Chemistry
Vesicle
Population
Blotting, Western
Clathrin-Coated Vesicles
Cell Fraction
biology.organism_classification
Cell Fractionation
Clathrin
General Biochemistry, Genetics and Molecular Biology
Mass Spectrometry
HeLa
Blot
Microscopy, Electron
Cell culture
RNA interference
biology.protein
Biophysics
Humans
education
HeLa Cells
Subjects
Details
- ISSN :
- 15596095
- Volume :
- 2014
- Issue :
- 11
- Database :
- OpenAIRE
- Journal :
- Cold Spring Harbor protocols
- Accession number :
- edsair.doi.dedup.....cb124ed6e295e44a96f128323baf54a2