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In vivo echocardiographic detection of enhanced left ventricular function in gene-targeted mice with phospholamban deficiency

Authors :
Brian D. Hoit
Evangelia G. Kranias
Saeb F. Khoury
Richard A. Walsh
Nancy Ball
Source :
Circulation research. 77(3)
Publication Year :
1995

Abstract

Abstract We evaluated the ability of M-mode and Doppler echocardiography to assess left ventricular (LV) function reliably and repeatedly in mice and tested whether these techniques could detect physiological alterations in phospholamban (PLB)–deficient mice. Anesthetized wild-type mice (n=7) and mice deficient in PLB (n=8) were studied with two-dimensional guided M-mode and Doppler echocardiography using a 9-MHz imaging and 5- to 7.5-MHz Doppler transducer. Data were acquired in the baseline state and after intraperitoneal isoproterenol administration (2.0 μg/g IP). Interobserver and intraobserver variability and reproducibility were excellent. PLB-deficient mice were associated with significant ( P 2 ), and peak early-diastolic transmitral velocity (80.0±7.2 versus 66.9±7.7 cm/s). LV dimensions, shortening fractions, heart rates, late diastolic transmitral (A) velocities, and early to late (E/A) diastolic velocity ratios were similar in both groups. Isoproterenol administration resulted in significant increases in Doppler indices of ventricular function in control but not PLB-deficient mice. These findings indicate that assessment of LV function can be performed noninvasively in mice under varying physiological conditions and that PLB regulates basal LV function in vivo.

Details

ISSN :
00097330
Volume :
77
Issue :
3
Database :
OpenAIRE
Journal :
Circulation research
Accession number :
edsair.doi.dedup.....cb1124887226a827002344ff7a1e28ce