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In vivo echocardiographic detection of enhanced left ventricular function in gene-targeted mice with phospholamban deficiency
- Source :
- Circulation research. 77(3)
- Publication Year :
- 1995
-
Abstract
- Abstract We evaluated the ability of M-mode and Doppler echocardiography to assess left ventricular (LV) function reliably and repeatedly in mice and tested whether these techniques could detect physiological alterations in phospholamban (PLB)–deficient mice. Anesthetized wild-type mice (n=7) and mice deficient in PLB (n=8) were studied with two-dimensional guided M-mode and Doppler echocardiography using a 9-MHz imaging and 5- to 7.5-MHz Doppler transducer. Data were acquired in the baseline state and after intraperitoneal isoproterenol administration (2.0 μg/g IP). Interobserver and intraobserver variability and reproducibility were excellent. PLB-deficient mice were associated with significant ( P 2 ), and peak early-diastolic transmitral velocity (80.0±7.2 versus 66.9±7.7 cm/s). LV dimensions, shortening fractions, heart rates, late diastolic transmitral (A) velocities, and early to late (E/A) diastolic velocity ratios were similar in both groups. Isoproterenol administration resulted in significant increases in Doppler indices of ventricular function in control but not PLB-deficient mice. These findings indicate that assessment of LV function can be performed noninvasively in mice under varying physiological conditions and that PLB regulates basal LV function in vivo.
- Subjects :
- Male
medicine.medical_specialty
Physiology
Diastole
Doppler echocardiography
Ventricular Function, Left
Contractility
Basal (phylogenetics)
symbols.namesake
Mice
In vivo
Internal medicine
medicine
Animals
medicine.diagnostic_test
business.industry
Calcium-Binding Proteins
Isoproterenol
Phospholamban
Mice, Inbred C57BL
Endocrinology
Echocardiography
Circulatory system
Gene Targeting
Cardiology
symbols
Female
Cardiology and Cardiovascular Medicine
business
Doppler effect
Subjects
Details
- ISSN :
- 00097330
- Volume :
- 77
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Circulation research
- Accession number :
- edsair.doi.dedup.....cb1124887226a827002344ff7a1e28ce