Back to Search
Start Over
Selective abdominal venous congestion to investigate cardiorenal interactions in a rat model
- Source :
- PLoS ONE, PLoS ONE, Vol 13, Iss 5, p e0197687 (2018)
- Publication Year :
- 2018
- Publisher :
- Public Library of Science, 2018.
-
Abstract
- Abdominal congestion may play an important role in the cardiorenal syndrome and has been demonstrated to drive disease progression. An animal model for abdominal congestion, without other culprit mechanisms that are often present in patients such as low cardiac output or chronic kidney disease, might be interesting to allow a better study of the pathophysiology of the cardiorenal syndrome. The objective of this study was to develop a clinically relevant and valid rat model with abdominal venous congestion and without pre-existing heart and/or kidney dysfunction. To do so, a permanent surgical constriction (20 Gauge) of the thoracic inferior vena cava (IVC) was applied in male Sprague Dawley rats (IVCc, n = 7), which were compared to sham-operated rats (SHAM, n = 6). Twelve weeks after surgery, abdominal venous pressure (mean: 13.8 vs 4.9 mmHg, p < 0.01), plasma creatinine (p < 0.05), plasma cystatin c (p < 0.01), urinary albumin (p < 0.05), glomerular surface area (p < 0.01) and width of Bowman's space (p < 0.05) of the IVCc group were significantly increased compared to the SHAM group for a comparable absolute body weight between groups (559 vs 530g, respectively, p = 0.73). Conventional cardiac echocardiographic and hemodynamic parameters did not differ significantly between both groups, indicating that cardiac function was not compromised by the surgery. In conclusion, we demonstrate that constriction of the thoracic IVC in adult rats is feasible and significantly increases the abdominal venous pressure to a clinically relevant level, thereby inducing abdominal venous congestion. J.C. is supported by BOF funding from UHasselt/BIOMED. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
- Subjects :
- 0301 basic medicine
Cystatins/blood
Male
Cardiac output
lcsh:Medicine
Hemodynamics
030204 cardiovascular system & hematology
Biochemistry
Rats, Sprague-Dawley
0302 clinical medicine
Anesthesiology
Medicine and Health Sciences
echocardiography
Anesthesia
lcsh:Science
Multidisciplinary
biology
Pharmaceutics
Hematology
Chemistry
medicine.vein
Creatinine
Physical Sciences
Inferior Vena Cava
Cardiology
Disease Progression
Anatomy
Glomeruli
Cardiology and Cardiovascular Medicine
Research Article
Chemical Elements
Cardiac function curve
medicine.medical_specialty
Surgical and Invasive Medical Procedures
Hyperemia
Vena Cava, Inferior
Cardiorenal syndrome
Inferior vena cava
Veins
Constriction
RATS
03 medical and health sciences
Drug Therapy
Albumins
Internal medicine
medicine
Animals
Hyperemia/complications
Cardio-Renal Syndrome/diagnostic imaging
Vena Cava, Inferior/physiopathology
Cardio-Renal Syndrome
business.industry
lcsh:R
Biology and Life Sciences
Kidneys
Renal System
Creatinine/blood
medicine.disease
Cystatins
Oxygen
Disease Models, Animal
030104 developmental biology
Cystatin C
Cardiovascular Anatomy
biology.protein
Blood Vessels
lcsh:Q
business
Albumins/metabolism
Venous Pressure
Biomarkers
Kidney disease
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- PLoS ONE, PLoS ONE, Vol 13, Iss 5, p e0197687 (2018)
- Accession number :
- edsair.doi.dedup.....cafb2e05eaaddfa09d80d85d50cec891