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Automated 3D-Printed Microfluidic Array for Rapid Nanomaterial-Enhanced Detection of Multiple Proteins

Authors :
Spundana Malla
Karteek Kadimisetty
Islam M. Mosa
Ketki S. Bhalerao
Norman H. Lee
James F. Rusling
Snehasis Bhakta
Source :
Analytical Chemistry. 90:7569-7577
Publication Year :
2018
Publisher :
American Chemical Society (ACS), 2018.

Abstract

We report here the fabrication and validation of a novel 3D-printed, automated immunoarray to detect multiple proteins with ultralow detection limits. This low cost, miniature immunoarray employs electrochemiluminescent (ECL) detection measured with a CCD camera and employs touch-screen control of a micropump to facilitate automated use. The miniaturized array features prefilled reservoirs to deliver sample and reagents to a paper-thin pyrolytic graphite microwell detection chip to complete sandwich immunoassays. The detection chip achieves high sensitivity by using single-wall carbon nanotube—antibody conjugates in the microwells and employing massively labeled antibody-decorated RuBPY—silica nanoparticles to generate ECL. The total cost of an array is $0.65, and an eight-protein assay can be done in duplicate for $0.14 per protein with limits of detection (LOD) as low as 78–110 fg mL(−1) in diluted serum. The electronic control system costs $210 in components. Utility of the automated immunoarray was demonstrated by detecting an eight-protein prostate cancer biomarker panel in human serum samples in 25 min. The system is well suited to future clinical and point-of-care diagnostic testing and could be used in resource-limited environments.

Details

ISSN :
15206882 and 00032700
Volume :
90
Database :
OpenAIRE
Journal :
Analytical Chemistry
Accession number :
edsair.doi.dedup.....caefc4864b0de34d255df80766873de2
Full Text :
https://doi.org/10.1021/acs.analchem.8b01198