Back to Search
Start Over
Antigenicity and immunogenicity of Plasmodium vivax merozoite surface protein-3
- Source :
- PLoS ONE, Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual), Universidade de São Paulo (USP), instacron:USP, PLoS ONE, Vol 8, Iss 2, p e56061 (2013)
- Publication Year :
- 2016
- Publisher :
- Public Library of Science, 2016.
-
Abstract
- A recent clinical trial in African children demonstrated the potential utility of merozoite surface protein (MSP)-3 as a vaccine against Plasmodium falciparum malaria. The present study evaluated the use of Plasmodium vivax MSP-3 (PvMSP-3) as a target antigen in vaccine formulations against malaria caused by P. vivax. Recombinant proteins representing MSP-3α and MSP-3β of P. vivax were expressed as soluble histidine-tagged bacterial fusions. Antigenicity during natural infection was evaluated by detecting specific antibodies using sera from individuals living in endemic areas of Brazil. A large proportion of infected individuals presented IgG antibodies to PvMSP-3α (68.2%) and at least 1 recombinant protein representing PvMSP-3β (79.1%). In spite of the large responder frequency, reactivity to both antigens was significantly lower than was observed for the immunodominant epitope present on the 19-kDa C-terminal region of PvMSP-1. Immunogenicity of the recombinant proteins was studied in mice in the absence or presence of different adjuvant formulations. PvMSP-3β, but not PvMSP-3α, induced a TLR4-independent humoral immune response in the absence of any adjuvant formulation. The immunogenicity of the recombinant antigens were also tested in formulations containing different adjuvants (Alum, Salmonella enterica flagellin, CpG, Quil A,TiterMax® and incomplete Freunds adjuvant) and combinations of two adjuvants (Alum plus flagellin, and CpG plus flagellin). Recombinant PvMSP-3α and PvMSP-3β elicited higher antibody titers capable of recognizing P. vivax-infected erythrocytes harvested from malaria patients. Our results confirm that P. vivax MSP-3 antigens are immunogenic during natural infection, and the corresponding recombinant proteins may be useful in elucidating their vaccine potential.
- Subjects :
- Epidemiology
medicine.medical_treatment
Plasmodium vivax
Protozoan Proteins
lcsh:Medicine
Protozoology
Mice
0302 clinical medicine
Plasmodium Vivax
Merozoite surface protein
lcsh:Science
Immune Response
Mice, Inbred BALB C
0303 health sciences
Multidisciplinary
biology
Immunogenicity
Vaccination
Recombinant Proteins
3. Good health
Infectious Diseases
Medicine
Adjuvant
Research Article
Antigenicity
Immunology
030231 tropical medicine
Antigens, Protozoan
Microbiology
03 medical and health sciences
Antigen
Malaria Vaccines
Vaccine Development
parasitic diseases
Malaria, Vivax
Parasitic Diseases
medicine
Animals
Humans
PLASMODIUM
Biology
030304 developmental biology
lcsh:R
Immunity
Tropical Diseases (Non-Neglected)
Plasmodium falciparum
biology.organism_classification
Virology
Malaria
Mice, Inbred C57BL
Immunoglobulin G
Antibody Formation
biology.protein
Parastic Protozoans
lcsh:Q
Clinical Immunology
Flagellin
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- PLoS ONE, Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual), Universidade de São Paulo (USP), instacron:USP, PLoS ONE, Vol 8, Iss 2, p e56061 (2013)
- Accession number :
- edsair.doi.dedup.....cad8c3b3820492f4e4c55627c83d8f30