Back to Search
Start Over
Another look at follicular lymphoma: immunophenotypic and molecular analyses identify distinct follicular lymphoma subgroups
- Source :
- Histopathology. 62(6)
- Publication Year :
- 2012
-
Abstract
- Aims: The aim of this study was to analyse the immunophenotypic and molecular features of a large series of follicular lymphomas, focusing in particular on atypical cases that fail to express CD10 and/or bcl-2. Such cases present diagnostic pitfalls, especially with regard to the differential diagnosis from follicular hyperplasia and marginal zone B-cell lymphoma. Therefore, we also included an immunohistochemical evaluation of stathmin, which is strongly expressed by germinal centre B cells, as a putative new marker for follicular lymphomas, particularly those with an atypical phenotype. Methods and results: Two hundred and five follicular lymphomas were investigated with immunohistochemistry and fluorescence in-situ hybridization (FISH). The use of three distinct anti-bcl-2 antibodies together with CD10 expression data and FISH analysis for bcl-2 and bcl-6 rearrangements allowed subclassification of follicular lymphoma into four distinct subgroups: (i) CD10-positive/bcl-2-positive, (ii) CD10-positive/bcl-2-negative, (iii) CD10-negative/bcl-2-positive, and (iv) CD10-negative/bcl-2-negative. All cases were bcl-6-positive. STMN1 (stathmin) was shown to be helpful in diagnosing bcl-2-negative and/or CD10-negative follicular lymphomas, and in their distinction from marginal zone B-cell lymphoma. Conclusions: Combined immunohistological and molecular analyses reveal that follicular lymphomas showing an atypical immunophenotypic and molecular profile exist, and we demonstrate that STMN1 represents a novel useful diagnostic marker for these. © 2013 Blackwell Publishing Ltd.
- Subjects :
- Adult
Male
Pathology
medicine.medical_specialty
Histology
phenotype
bcl-2
Follicular lymphoma
Biology
Pathology and Forensic Medicine
Immunophenotyping
follicular lymhpoma
Diagnosis, Differential
Young Adult
FISH
immune system diseases
hemic and lymphatic diseases
Follicular phase
medicine
Biomarkers, Tumor
Humans
music
Lymphoma, Follicular
B cell
In Situ Hybridization, Fluorescence
Aged
Aged, 80 and over
Gene Rearrangement
music.instrument
General Medicine
Gene rearrangement
Lymphoma, B-Cell, Marginal Zone
Middle Aged
medicine.disease
Marginal zone
Follicular hyperplasia
Lymphoma
Genes, bcl-2
DNA-Binding Proteins
medicine.anatomical_structure
Proto-Oncogene Proteins c-bcl-2
immunohistochemistry
Proto-Oncogene Proteins c-bcl-6
Stathmin
Female
Neprilysin
Subjects
Details
- ISSN :
- 13652559
- Volume :
- 62
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- Histopathology
- Accession number :
- edsair.doi.dedup.....cad52109c4209b1d69216ada7bbac5b2