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Another look at follicular lymphoma: immunophenotypic and molecular analyses identify distinct follicular lymphoma subgroups

Authors :
Josette Brière
Pierre Sujobert
Corinne Haioun
Peter G. Isaacson
William Townsend
Asim Khwaja
Hongxiang Liu
David C. Linch
Christiane Copie-Bergman
Thomas M. Grogan
Jennifer C. Paterson
Teresa Marafioti
Andrew Clear
Alan D. Ramsay
Stefano Pileri
Philippe Gaulard
Claudio Agostinelli
Vishvesh H. Shende
Noraidah Masir
Maryse Baia
Kandavel Shanmugam
Elizabeth Nacheva
Ming-Qing Du
Kirit M. Ardeshna
Maria Calaminici
Pier Paolo Piccaluga
John G. Gribben
Simon P. Brooks
Teresa Marafioti
Christiane Copie-Bergman
Maria Calaminici
Jennifer C Paterson
Vishvesh H Shende
Hongxiang Liu
Maryse Baia
Alan D Ramsay
Claudio Agostinelli
Josette Brière
Andrew Clear
Ming-Qing Du
Pier Paolo Piccaluga
Noraidah Masir
Elizabeth P Nacheva
Pierre Sujobert
Kandavel Shanmugam
Thomas M Grogan
Simon P Brook
Asim Khwaja
Kirit Ardeshna
William Townsend
Stefano A Pileri
Corinne Haioun
David Linch
John G Gribben
Philippe Gaulard
Peter G Isaacson
Source :
Histopathology. 62(6)
Publication Year :
2012

Abstract

Aims: The aim of this study was to analyse the immunophenotypic and molecular features of a large series of follicular lymphomas, focusing in particular on atypical cases that fail to express CD10 and/or bcl-2. Such cases present diagnostic pitfalls, especially with regard to the differential diagnosis from follicular hyperplasia and marginal zone B-cell lymphoma. Therefore, we also included an immunohistochemical evaluation of stathmin, which is strongly expressed by germinal centre B cells, as a putative new marker for follicular lymphomas, particularly those with an atypical phenotype. Methods and results: Two hundred and five follicular lymphomas were investigated with immunohistochemistry and fluorescence in-situ hybridization (FISH). The use of three distinct anti-bcl-2 antibodies together with CD10 expression data and FISH analysis for bcl-2 and bcl-6 rearrangements allowed subclassification of follicular lymphoma into four distinct subgroups: (i) CD10-positive/bcl-2-positive, (ii) CD10-positive/bcl-2-negative, (iii) CD10-negative/bcl-2-positive, and (iv) CD10-negative/bcl-2-negative. All cases were bcl-6-positive. STMN1 (stathmin) was shown to be helpful in diagnosing bcl-2-negative and/or CD10-negative follicular lymphomas, and in their distinction from marginal zone B-cell lymphoma. Conclusions: Combined immunohistological and molecular analyses reveal that follicular lymphomas showing an atypical immunophenotypic and molecular profile exist, and we demonstrate that STMN1 represents a novel useful diagnostic marker for these. © 2013 Blackwell Publishing Ltd.

Details

ISSN :
13652559
Volume :
62
Issue :
6
Database :
OpenAIRE
Journal :
Histopathology
Accession number :
edsair.doi.dedup.....cad52109c4209b1d69216ada7bbac5b2