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Optimization of Degradation Conditions with PRG, a Polysaccharide from Phellinus ribis, by RSM and the Neuroprotective Activity in PC12 Cells Damaged by Aβ25–35
- Source :
- Molecules, Volume 24, Issue 16, Molecules, Vol 24, Iss 16, p 3010 (2019)
- Publication Year :
- 2019
- Publisher :
- MDPI, 2019.
-
Abstract
- In the previous work, we found PRG, a polysaccharide from Phellinus ribis, exhibited neurotrophic activity. To obtain an active structural unit with lower molecular weight, PRG was degraded to prepare the degraded PRG (DPRG) using ascorbic acid and H2O2. The aim of the paper was to obtain DPRG by optimizing the degradation conditions using response surface methodology (RSM) and to study its protective effects of PC12 cells induced by A&beta<br />25&ndash<br />35. The optimum conditions were as follows<br />the concentration of H2O2-Vc was 17 mM and degradation temperature was 50 &deg<br />C<br />when degradation time was 1.6 h, the experimental response value of PC12 cell viability was 83.4 &plusmn<br />0.15%, which was in accordance with the predicted value (83.5%). We also studied the protective effects of DPRG against the A&beta<br />35-induced neurotoxicity and explored the underlying mechanism. The results showed that treatment with DPRG could attenuate PC12 cells death. The mechanism was relative to the inhibition of cell apoptosis by increasing the MMP level and decreasing the protein expression of cytochrome C (Cytc) in PC12 cells. In conclusion, DPRG with lower molecular weight was obtained successfully. It possessed neuroprotective properties and might be a candidate for neurodegenerative disease treatment.
- Subjects :
- Pharmaceutical Science
Apoptosis
02 engineering and technology
PC12 Cells
Analytical Chemistry
Drug Discovery
Spectroscopy, Fourier Transform Infrared
degradation
chemistry.chemical_classification
Membrane Potential, Mitochondrial
Neurons
0303 health sciences
biology
Chemistry
Cytochrome c
Temperature
021001 nanoscience & nanotechnology
Mitochondria
Neuroprotective Agents
Biochemistry
Chemistry (miscellaneous)
Molecular Medicine
0210 nano-technology
Cell Survival
amyloid-β
Polysaccharide
Neuroprotection
Article
lcsh:QD241-441
03 medical and health sciences
mitochondrial membrane permeability
cytochrome C
lcsh:Organic chemistry
Phellinus ribis
medicine
Animals
Response surface methodology
Viability assay
Physical and Theoretical Chemistry
030304 developmental biology
Amyloid beta-Peptides
Basidiomycota
Organic Chemistry
Neurotoxicity
Proteins
Fungal Polysaccharides
Hydrogen Peroxide
Ascorbic acid
medicine.disease
neuroprotevtive activity
Peptide Fragments
Rats
Molecular Weight
polysaccharide
biology.protein
Subjects
Details
- Language :
- English
- ISSN :
- 14203049
- Volume :
- 24
- Issue :
- 16
- Database :
- OpenAIRE
- Journal :
- Molecules
- Accession number :
- edsair.doi.dedup.....cad18de9fe831f449917cc284f8e273f