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Growth factors, kinetics and biodegradation mechanism associated with Pseudomonas nitroreducens TX1 grown on octylphenol polyethoxylates
- Source :
- Journal of environmental management. 80(4)
- Publication Year :
- 2004
-
Abstract
- The growth properties and biodegradation mechanism of a Gram-negative bacterium, Pseudomonas nitroreducens TX1 that was able to grow on branched octylphenol polyethoxylates (OPEO(n), average n=9.5) as the sole carbon source over a wide concentration range (1-100,000 mgl(-1)) were studied. Analysis of growth factors indicated the highest specific growth rate (micro) of 0.53 h(-1) was obtained at an initial concentration of 5,000 mgl(-1) OPEO(n). An optimal C/N ratio of 12 was obtained for (NH(4))(2)SO(4) as the nitrogen source in a cultivated medium at pH 7. The kinetic analysis demonstrated that bacterial growth and OPEO(n) degradation followed the Monod equation and were based on a substrate concentration inhibition model and pseudo-first-order reaction, respectively. The substrate inhibition coefficient was over 18,000 mgl(-1) and this indicates that the strain has an ability to sustain growth at high concentrations of OPEO(n) and use it as the sole carbon source under such a stress condition. Furthermore, LC-MS analysis showed that the biodegradation mechanism of dodecyl octaethoxylate (AEO8) by P. nitroreducens TX1 was the sequential cleavage of the ethoxylate chain.
- Subjects :
- Environmental Engineering
Nitrogen
Polymers
Management, Monitoring, Policy and Law
Bacterial growth
Mass Spectrometry
Piperidines
Pseudomonas
Monod equation
Waste Management and Disposal
Cell Proliferation
Chromatography
biology
Molecular Structure
Chemistry
Substrate (chemistry)
General Medicine
Pseudomonas nitroreducens
Biodegradation
Hydrogen-Ion Concentration
biology.organism_classification
Kinetics
Pseudomonadales
Intercellular Signaling Peptides and Proteins
Pseudomonadaceae
Subjects
Details
- ISSN :
- 03014797
- Volume :
- 80
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Journal of environmental management
- Accession number :
- edsair.doi.dedup.....cab0718af3530e30131b515b38d2cd4f