Back to Search
Start Over
pH (low) insertion peptide (pHLIP) targets ischemic myocardium
- Publication Year :
- 2012
- Publisher :
- Columbia University, 2012.
-
Abstract
- The pH (low) insertion peptide (pHLIP) family enables targeting of cells in tissues with low extracellular pH. Here, we show that ischemic myocardium is targeted, potentially opening a new route to diagnosis and therapy. The experiments were performed using two murine ischemia models: regional ischemia induced by coronary artery occlusion and global low-flow ischemia in isolated hearts. In both models, pH-sensitive pHLIPs [wild type (WT) and Var7] or WT-pHLIP–coated liposomes bind ischemic but not normal regions of myocardium, whereas pH-insensitive, kVar7, and liposomes coated with PEG showed no preference. pHLIP did not influence either the mechanical or the electrical activity of ischemic myocardium. In contrast to other known targeting strategies, the pHLIP-based binding does not require severe myocardial damage. Thus, pHLIP could be used for delivery of pharmaceutical agents or imaging probes to the myocardial regions undergoing brief restrictions of blood supply that do not induce irreversible changes in myocytes.
- Subjects :
- Male
Pathology
medicine.medical_specialty
Ischemic myocardium
Ischemia
Myocardial Ischemia
Peptide
Pharmacology
Fluorescence
Mice
Drug Delivery Systems
PEG ratio
medicine
Extracellular
Myocyte
Animals
chemistry.chemical_classification
Liposome
Multidisciplinary
business.industry
Myocardium
FOS: Clinical medicine
Wild type
Neurosciences
Membrane Proteins
Biological Sciences
Hydrogen-Ion Concentration
medicine.disease
Mice, Inbred C57BL
chemistry
Liposomes
business
Cytology
Subjects
Details
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....caaf97f9111c779b7252f0718e5c6413
- Full Text :
- https://doi.org/10.7916/d8154t2d