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Application of an UHPLC–MS/MS method to tissue distribution and excretion study of 2-(2-hydroxypropanamido) benzoic acid in rats
- Source :
- Journal of Chromatography B. 1070:54-61
- Publication Year :
- 2017
- Publisher :
- Elsevier BV, 2017.
-
Abstract
- s 2-(2-Hydroxypropanamido) benzoic acid (HPABA) is a potential anti-inflammatory agent isolated from the marine fungus Penicillium chrysogenum . In vivo tissue distribution and excretion of HPABA were unknown. Therefore, the purpose of this paper was to establish an UHPLC–MS/MS method for the determination of HPABA and proceed to apply for the study of tissue distribution and excretion in rats. The results obtained indicated that HPABA in tissues was rapidly distributed and cleared. The highest level was found in intestine and stomach, followed by kidney, liver, lung and heart. These observations showed that HPABA was well distributed into abundant blood-supply tissues, suggesting that the blood flow or perfusion rate of organs plays a major role. A small amount of HPABA was detected in brain indicated that the ability of it in crossing the blood brain barrier (BBB) was very weak in rats. Excretion studies demonstrated that HPABA was mainly excreted by kidney. The total cumulative excretion of HPABA were 54.3% and 44.1% in male and female rats, respectively. In addition, gender-related differences in the excretion and distribution profile of HPABA were observed in rats.
- Subjects :
- Male
medicine.medical_specialty
Clinical Biochemistry
Penicillium chrysogenum
Blood–brain barrier
Benzoates
Sensitivity and Specificity
01 natural sciences
Biochemistry
Analytical Chemistry
Excretion
chemistry.chemical_compound
Drug Stability
Tandem Mass Spectrometry
In vivo
Internal medicine
medicine
Animals
Distribution (pharmacology)
Tissue Distribution
Chromatography, High Pressure Liquid
Benzoic acid
Kidney
Chromatography
biology
Stomach
010401 analytical chemistry
Reproducibility of Results
Cell Biology
General Medicine
biology.organism_classification
Rats
0104 chemical sciences
010404 medicinal & biomolecular chemistry
Endocrinology
medicine.anatomical_structure
chemistry
Linear Models
Female
Subjects
Details
- ISSN :
- 15700232
- Volume :
- 1070
- Database :
- OpenAIRE
- Journal :
- Journal of Chromatography B
- Accession number :
- edsair.doi.dedup.....caadf0787547dff6c0f5f9aefc8df32d