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Fabrication of KR-12 peptide-containing hyaluronic acid immobilized fibrous eggshell membrane effectively kills multi-drug-resistant bacteria, promotes angiogenesis and accelerates re-epithelialization
- Source :
- International Journal of Nanomedicine
- Publication Year :
- 2019
- Publisher :
- Informa UK Limited, 2019.
-
Abstract
- Menglong Liu,1 Tengfei Liu,1 Xiaorong Zhang,1 Zhiwen Jian,2 Hesheng Xia,2 Jiacai Yang,1 Xiaohong Hu,1 Malcolm Xing,1,3 Gaoxing Luo,1 Jun Wu1,41Institute of Burn Research, State Key Laboratory of Trauma, Burn and Combined Injury, Southwest Hospital, Third Military Medical University (Army Medical University), Chongqing 400038, People’s Republic of China; 2State Key Laboratory of Polymer Materials Engineering, Polymer Research Institute of Sichuan University, Chengdu, 610065, People’s Republic of China; 3Department of Mechanical Engineering, University of Manitoba, Winnipeg, MB R3T 2N2, Canada; 4Department of Burns, the First Affiliated Hospital, SunYat-Sen University, Guangzhou 510080, People’s Republic of ChinaBackground: Designing a wound dressing that effectively prevents multi-drug-resistant bacterial infection and promotes angiogenesis and re-epithelialization is of great significance for wound management.Methods and results: In this study, a biocompatible composite membrane comprising biomimetic polydopamine-modified eggshell membrane nano/microfibres coated with KR-12 antimicrobial peptide and hyaluronic acid (HA) was developed in an eco-friendly manner. The physicochemical properties of the composite membrane were thoroughly characterized, and the results showed that the surface hydrophilicity and water absorption ability of the composite membrane were improved after the successive conjugation of the HA and the KR-12 peptide. Furthermore, the in vitrobiological results revealed that the composite membrane had excellent antibacterial activity against Gram-positive Staphylococcus aureus, methicillin-resistant Staphylococcus aureus (MRSA) and Gram-negative Escherichia coli, and it could prevent MRSA biofilm formation on its surface. Additionally, it promoted the proliferation of keratinocytes and human umbilical vein endothelial cells and increased the secretion of VEGF. Finally, an in vivo animal study indicated that the composite membrane could promote wound healing via accelerating angiogenesis and re-epithelialization, which were demonstrated by the enhanced expression of angiogenetic markers (CD31 and VEGF) and keratinocyte proliferation marker (PCNA), respectively.Conclusion: These results indicated that the composite membrane is a potential candidate of wound dressingsKeywords: antimicrobial peptides, hyaluronic acid, fibrous eggshell membrane, antibacterial activity, angiogenesis, re-epithelialization
- Subjects :
- Keratinocytes
Angiogenesis
Pharmaceutical Science
02 engineering and technology
01 natural sciences
antimicrobial peptides
angiogenesis
Egg Shell
Mice
chemistry.chemical_compound
antibacterial activity
Re-Epithelialization
International Journal of Nanomedicine
Drug Resistance, Multiple, Bacterial
hyaluronic acid
Drug Discovery
Hyaluronic acid
fibrous eggshell membrane
Original Research
General Medicine
021001 nanoscience & nanotechnology
Anti-Bacterial Agents
Cell biology
medicine.anatomical_structure
0210 nano-technology
Keratinocyte
Porosity
Methicillin-Resistant Staphylococcus aureus
Staphylococcus aureus
Antimicrobial peptides
Biophysics
Neovascularization, Physiologic
Bioengineering
Microbial Sensitivity Tests
010402 general chemistry
Biomaterials
In vivo
Escherichia coli
Human Umbilical Vein Endothelial Cells
medicine
Animals
Humans
Secretion
Organic Chemistry
0104 chemical sciences
chemistry
Eggshell membrane
Peptides
Wound healing
Chickens
Subjects
Details
- ISSN :
- 11782013
- Volume :
- 14
- Database :
- OpenAIRE
- Journal :
- International Journal of Nanomedicine
- Accession number :
- edsair.doi.dedup.....caa0462507aaf06873f71a964f7054f2