Back to Search
Start Over
Mechanical loading regulates NOS expression and activity in developing and adult skeletal muscle
- Source :
- The American journal of physiology. 275(1)
- Publication Year :
- 1998
-
Abstract
- The hypothesis that changes in muscle activation and loading regulate the expression and activity of neuronal nitric oxide (NO) synthase (nNOS) was tested using in vitro and in vivo approaches. Removal of weight bearing from rat hindlimb muscles for 10 days resulted in a significant decrease in nNOS protein and mRNA concentration in soleus muscles, which returned to control concentrations after return to weight bearing. Similarly, the concentration of nNOS in cultured myotubes increased by application of cyclic loading for 2 days. NO release from excised soleus muscles was increased significantly by a single passive stretch of 20% or by submaximal activation at 2 Hz, although the increases were not additive when both stimuli were applied simultaneously. Increased NO release resulting from passive stretch or activation was dependent on the presence of extracellular calcium. Cyclic loading of cultured myotubes also resulted in a significant increase in NO release. Together, these findings show that activity of muscle influences NO production in the short term, by regulating NOS activity, and in the long term, by regulating nNOS expression.
- Subjects :
- medicine.medical_specialty
Aging
Time Factors
Transcription, Genetic
Physiology
Movement
chemistry.chemical_element
Hindlimb
Nitric Oxide Synthase Type I
Calcium
Motor Activity
Muscle Development
Gene Expression Regulation, Enzymologic
Internal medicine
Myosin
medicine
Extracellular
Animals
RNA, Messenger
Rats, Wistar
Muscle, Skeletal
Cells, Cultured
biology
Myosin Heavy Chains
Myogenesis
Skeletal muscle
Gene Expression Regulation, Developmental
Cell Biology
Electric Stimulation
Rats
Nitric oxide synthase
medicine.anatomical_structure
Endocrinology
chemistry
Hindlimb Suspension
Protein Biosynthesis
biology.protein
Female
Stress, Mechanical
medicine.symptom
Nitric Oxide Synthase
Muscle contraction
Subjects
Details
- ISSN :
- 00029513
- Volume :
- 275
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- The American journal of physiology
- Accession number :
- edsair.doi.dedup.....ca9d363dadca83699943714abe8b80cb