Back to Search
Start Over
Structural insight into DNA joining: from conserved mechanisms to diverse scaffolds
- Source :
- Nucleic Acids Research
- Publication Year :
- 2020
- Publisher :
- Oxford University Press (OUP), 2020.
-
Abstract
- DNA ligases are diverse enzymes with essential functions in replication and repair of DNA; here we review recent advances in their structure and distribution and discuss how this contributes to understanding their biological roles and technological potential. Recent high-resolution crystal structures of DNA ligases from different organisms, including DNA-bound states and reaction intermediates, have provided considerable insight into their enzymatic mechanism and substrate interactions. All cellular organisms possess at least one DNA ligase, but many species encode multiple forms some of which are modular multifunctional enzymes. New experimental evidence for participation of DNA ligases in pathways with additional DNA modifying enzymes is defining their participation in non-redundant repair processes enabling elucidation of their biological functions. Coupled with identification of a wealth of DNA ligase sequences through genomic data, our increased appreciation of the structural diversity and phylogenetic distribution of DNA ligases has the potential to uncover new biotechnological tools and provide new treatment options for bacterial pathogens.
- Subjects :
- Models, Molecular
chemistry.chemical_classification
DNA ligase
Genome
VDP::Mathematics and natural science: 400::Chemistry: 440
DNA Ligases
AcademicSubjects/SCI00010
Protein Conformation
Mechanism (biology)
Computational biology
Multifunctional Enzymes
Biology
ENCODE
Catalysis
Structure-Activity Relationship
chemistry.chemical_compound
Protein structure
chemistry
VDP::Matematikk og Naturvitenskap: 400::Kjemi: 440
Genetics
Humans
Survey and Summary
DNA
Subjects
Details
- ISSN :
- 13624962 and 03051048
- Volume :
- 48
- Database :
- OpenAIRE
- Journal :
- Nucleic Acids Research
- Accession number :
- edsair.doi.dedup.....ca89b8d90dfed2afd9139b6b2afadade