Back to Search
Start Over
The LOTUS domain is a conserved DEAD-box RNA helicase regulator essential for the recruitment of Vasa to the germ plasm and nuage
- Source :
- 'Genes & Development ', vol: 31, pages: 939-952 (2017)
- Publication Year :
- 2017
- Publisher :
- Cold Spring Harbor Laboratory, 2017.
-
Abstract
- DEAD-box RNA helicases play important roles in a wide range of metabolic processes. Regulatory proteins can stimulate or block the activity of DEAD-box helicases. Here, we show that LOTUS (Limkain, Oskar, and Tudor containing proteins 5 and 7) domains present in the germline proteins Oskar, TDRD5 (Tudor domain-containing 5), and TDRD7 bind and stimulate the germline-specific DEAD-box RNA helicase Vasa. Our crystal structure of the LOTUS domain of Oskar in complex with the C-terminal RecA-like domain of Vasa reveals that the LOTUS domain occupies a surface on a DEAD-box helicase not implicated previously in the regulation of the enzyme's activity. We show that, in vivo, the localization of Drosophila Vasa to the nuage and germ plasm depends on its interaction with LOTUS domain proteins. The binding and stimulation of Vasa DEAD-box helicases by LOTUS domains are widely conserved.
- Subjects :
- 0301 basic medicine
DEAD box
Protein Conformation
Lotus
Regulator
oskar
Gene Expression Regulation, Enzymologic
Germline
Animals, Genetically Modified
DEAD-box RNA Helicases
03 medical and health sciences
Protein Domains
Genetics
Animals
Drosophila Proteins
Cells, Cultured
Germ plasm
biology
urogenital system
fungi
food and beverages
Helicase
biology.organism_classification
RNA Helicase A
Germ Cells
030104 developmental biology
biology.protein
Drosophila
Female
Research Paper
Developmental Biology
Subjects
Details
- ISSN :
- 15495477 and 08909369
- Volume :
- 31
- Database :
- OpenAIRE
- Journal :
- Genes & Development
- Accession number :
- edsair.doi.dedup.....ca65eb3e210f2655803a0f9e0ea84f46