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The CCR4-NOT complex component NOT1 regulates RNA-directed DNA methylation and transcriptional silencing by facilitating Pol IV-dependent siRNA production
- Source :
- The Plant journal : for cell and molecular biologyREFERENCES. 103(4)
- Publication Year :
- 2020
-
Abstract
- Small interfering RNAs (siRNAs) are responsible for establishing and maintaining DNA methylation through the RNA-directed DNA methylation (RdDM) pathway in plants. Although siRNA biogenesis is well known, it is relatively unclear about how the process is regulated. By a forward genetic screen in Arabidopsis thaliana, we identified a mutant defective in NOT1 and demonstrated that NOT1 is required for transcriptional silencing at RdDM target genomic loci. We demonstrated that NOT1 is required for Pol IV-dependent siRNA accumulation and DNA methylation at a subset of RdDM target genomic loci. Furthermore, we revealed that NOT1 is a constituent of a multi-subunit CCR4-NOT deadenylase complex by immunoprecipitation combined with mass spectrometry and demonstrated that the CCR4-NOT components can function as a whole to mediate chromatin silencing. Therefore, our work establishes that the CCR4-NOT complex regulates the biogenesis of Pol IV-dependent siRNAs, and hence facilitates DNA methylation and transcriptional silencing in Arabidopsis.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Small interfering RNA
Arabidopsis
Chromatin silencing
Plant Science
01 natural sciences
03 medical and health sciences
Genetics
Gene silencing
RNA, Small Interfering
RNA-Directed DNA Methylation
biology
Arabidopsis Proteins
RNA
Cell Biology
DNA-Directed RNA Polymerases
DNA Methylation
biology.organism_classification
Cell biology
Repressor Proteins
030104 developmental biology
DNA methylation
010606 plant biology & botany
Genetic screen
Transcription Factors
Subjects
Details
- ISSN :
- 1365313X
- Volume :
- 103
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- The Plant journal : for cell and molecular biologyREFERENCES
- Accession number :
- edsair.doi.dedup.....ca5373d2ab16639bbb346febc71ccd19