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Selective 5-Lipoxygenase Expression in Langerhans Cells and Impaired Dendritic Cell Migration in 5-LO-Deficient Mice Reveal Leukotriene Action in Skin
- Source :
- Journal of Investigative Dermatology. 127(7):1692-1700
- Publication Year :
- 2007
- Publisher :
- Elsevier BV, 2007.
-
Abstract
- 5-Lipoxygenase (5-LO) catalyzes the initial steps in the formation of leukotrienes (LTs), which are implicated in immune reactions. Recently, it was shown that FITC-triggered epidermal Langerhans cell (LC) emigration to draining lymph nodes (LNs) is impaired in LTC4 export pump (multidrug resistance-associated protein 1)-deficient mice. Here, we sought genetic evidence for a role of endogenous LTs in dendritic cell function through the study of 5-LO-deficient mice. Though DC numbers in skin, spleen, and peripheral LNs were similar in both 5-LO-deficient and wild-type (WT) mice, DC homing from skin to draining LNs induced by FITC was reduced by 75% in 5-LO-deficient mice. Moreover, in WT mice, all epidermal LCs, dermal langerin+ LCs, and subsets of dermal macrophages and langerin+ LCs in T-cell areas of skin-draining LNs markedly expressed 5-LO. However, the enzyme was noticeably absent in all DC subsets of the dermis, thymus, spleen, Peyer's patches, mesenteric LNs, and mucosal surfaces of lung and intestine. As all epidermal cells other than LCs lacked 5-LO and because differentiation and activation of DCs generated from 5-LO-deficient mice in vitro were normal, these data support a selective role of endogenous LTs in DC homing following skin sensitization.
- Subjects :
- Leukotrienes
Langerhans cell
Langerin
Lymphoid Tissue
Spleen
Thymus Gland
Dermatology
Biochemistry
Gene Expression Regulation, Enzymologic
Mice
03 medical and health sciences
0302 clinical medicine
Dermis
Cell Movement
medicine
Animals
Dendritic cell migration
Antigen-presenting cell
Lung
Molecular Biology
030304 developmental biology
Mice, Knockout
0303 health sciences
Arachidonate 5-Lipoxygenase
biology
integumentary system
Cell Differentiation
Cell migration
hemic and immune systems
Dendritic Cells
Dendritic cell
Cell Biology
Molecular biology
Intestines
Mice, Inbred C57BL
medicine.anatomical_structure
Gene Expression Regulation
Langerhans Cells
Immunology
biology.protein
Fluorescein-5-isothiocyanate
030215 immunology
Subjects
Details
- ISSN :
- 0022202X
- Volume :
- 127
- Issue :
- 7
- Database :
- OpenAIRE
- Journal :
- Journal of Investigative Dermatology
- Accession number :
- edsair.doi.dedup.....ca4ebcce48f2976a3313392e87c7e536
- Full Text :
- https://doi.org/10.1038/sj.jid.5700796