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Stages, scaffolds and strings in the spatial organisation of non-homologous end joining: Insights from X-ray diffraction and Cryo-EM
- Source :
- Progress in Biophysics and Molecular Biology
- Publication Year :
- 2021
- Publisher :
- Elsevier BV, 2021.
-
Abstract
- Non-homologous end joining (NHEJ) is the preferred pathway for the repair of DNA double-strand breaks in humans. Here we describe three structural aspects of the repair pathway: stages, scaffolds and strings. We discuss the orchestration of DNA repair to guarantee robust and efficient NHEJ. We focus on structural studies over the past two decades, not only using X-ray diffraction, but also increasingly exploiting cryo-EM to investigate the macromolecular assemblies.
- Subjects :
- Materials science
DNA End-Joining Repair
Spatial organisation
DNA Repair
Cryo-electron microscopy
DNA repair
Double-strand breaks
030303 biophysics
Biophysics
KBM, Ku-binding motif
Computational biology
Article
03 medical and health sciences
chemistry.chemical_compound
DNA-PK, DNA-dependent protein kinase
PAXX, Paralog of XRCC4 and XLF
XLF, XRCC4-Like Factor
Stages
Strings
Humans
DSB, double-strand break
DNA Breaks, Double-Stranded
Orchestration (computing)
vWA, von Willebrand factor type A
cryo-EM, cryo-electron microscopy
PTIP, Pax transcription-activation-domain interacting protein
Molecular Biology
NHEJ
Cryo-EM
IR, ionising radiation
Scaffolds
0303 health sciences
53BP1, p53-binding protein 1
Cryoelectron Microscopy
X-ray diffraction
Non-homologous end joining
enzymes and coenzymes (carbohydrates)
chemistry
DNA-PKcs, DNA-PK catalytic subunit
NHEJ, non-homologous end joining
X-ray crystallography
PNKP, polynucleotide kinase
APLF, aprataxin and PNKP-like factor
BRCT, BRCA1 C-Terminus domain
lncRNA, long noncoding RNA
HR, homologous recombination
DNA
CTR, C-terminal region
Subjects
Details
- Database :
- OpenAIRE
- Journal :
- Progress in Biophysics and Molecular Biology
- Accession number :
- edsair.doi.dedup.....ca43e6e448ffae277c09ed2c9f19bd34