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GDNF and BDNF Alter the Expression of Neuronal NOS, c-Jun, and p75 and Prevent Motoneuron Death following Spinal Root Avulsion in Adult Rats
- Source :
- Journal of Neurotrauma. 20:603-612
- Publication Year :
- 2003
- Publisher :
- Mary Ann Liebert Inc, 2003.
-
Abstract
- In the present study, we examined the effects of glial cell line-derived neurotrophic factor (GDNF), brain-derived neurotrophic factor (BDNF), ciliary neurotrophic factor (CNTF), and insulin growth factor (IGF-1) on adult motoneuron survival following spinal root avulsion. The expression of neuronal nitric oxide synthase (nNOS), c-Jun, and the low-affinity neurotrophin receptor (P75) following treatment with these neurotrophic factors was also examined. In control animals, approximately 80% of spinal motoneurons were nNOS positive at 3 weeks following the lesion, whereas in GDNF or BDNF treated animals no nNOS positive motoneurons were found at the same time point. Following injury and treatment with GDNF and BDNF increased numbers of motoneurons were c-Jun and P75 positive. By 6 weeks following the lesion, only approximately 28% of motoneurons persisted in control animals whereas about 90% of motoneurons survived injury following treatment with either GDNF or BDNF. In contrast, CNTF and IGF-1 were ineffective in either inhibiting nNOS expression or preventing motoneuron death. Our results provide in vivo evidence that the survival of injured adult mammalian motoneurons can be promoted by specific neurotrophic factors, and that this effect is associated with inhibition of nNOS expression and up-regulation of c-Jun and P75 expression.
- Subjects :
- Male
medicine.medical_specialty
Proto-Oncogene Proteins c-jun
medicine.medical_treatment
Nitric Oxide Synthase Type I
Receptors, Nerve Growth Factor
Ciliary neurotrophic factor
Receptor, Nerve Growth Factor
Rats, Sprague-Dawley
Lesion
Neurotrophic factors
Internal medicine
Glial cell line-derived neurotrophic factor
medicine
Animals
Glial Cell Line-Derived Neurotrophic Factor
Nerve Growth Factors
Radiculopathy
Motor Neurons
Cell Death
biology
business.industry
Brain-Derived Neurotrophic Factor
c-jun
musculoskeletal system
Rats
Nitric oxide synthase
Endocrinology
Gene Expression Regulation
nervous system
biology.protein
Neurology (clinical)
Nitric Oxide Synthase
medicine.symptom
Axotomy
business
tissues
Neuroscience
Neurotrophin
Subjects
Details
- ISSN :
- 15579042 and 08977151
- Volume :
- 20
- Database :
- OpenAIRE
- Journal :
- Journal of Neurotrauma
- Accession number :
- edsair.doi.dedup.....ca15d81d74f5e82c66ac346cedcc1cd8