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Stable distinct core eukaryotic viromes in different mosquito species from Guadeloupe, using single mosquito viral metagenomics
- Source :
- Microbiome, Microbiome, BioMed Central, 2019, 7, pp.121. ⟨10.1186/s40168-019-0734-2⟩, Microbiome, 2019, 7, pp.121. ⟨10.1186/s40168-019-0734-2⟩, Microbiome, Vol 7, Iss 1, Pp 1-20 (2019)
- Publication Year :
- 2019
- Publisher :
- HAL CCSD, 2019.
-
Abstract
- Background Mosquitoes are the most important invertebrate viral vectors in humans and harbor a high diversity of understudied viruses, which has been shown in many mosquito virome studies in recent years. These studies generally performed metagenomics sequencing on pools of mosquitoes, without assessment of the viral diversity in individual mosquitoes. To address this issue, we applied our optimized viral metagenomics protocol (NetoVIR) to compare the virome of single and pooled Aedes aegypti and Culex quinquefasciatus mosquitoes collected from different locations in Guadeloupe, in 2016 and 2017. Results The total read number and viral reads proportion of samples containing a single mosquito have no significant difference compared with those of pools containing five mosquitoes, which proved the feasibility of using single mosquito for viral metagenomics. A comparative analysis of the virome revealed a higher abundance and more diverse eukaryotic virome in Aedes aegypti, whereas Culex quinquefasciatus harbors a richer and more diverse phageome. The majority of the identified eukaryotic viruses were mosquito-species specific. We further characterized the genomes of 11 novel eukaryotic viruses. Furthermore, qRT-PCR analyses of the six most abundant eukaryotic viruses indicated that the majority of individual mosquitoes were infected by several of the selected viruses with viral genome copies per mosquito ranging from 267 to 1.01 × 108 (median 7.5 × 106) for Ae. aegypti and 192 to 8.69 × 106 (median 4.87 × 104) for Cx. quinquefasciatus. Additionally, in Cx. quinquefasciatus, a number of phage contigs co-occurred with several marker genes of Wolbachia sp. strain wPip. Conclusions We firstly demonstrate the feasibility to use single mosquito for viral metagenomics, which can provide much more precise virome profiles of mosquito populations. Interspecific comparisons show striking differences in abundance and diversity between the viromes of Ae. aegypti and Cx. quinquefasciatus. Those two mosquito species seem to have their own relatively stable "core eukaryotic virome", which might have important implications for the competence to transmit important medically relevant arboviruses. The presence of Wolbachia in Cx. quinquefasciatus might explain (1) the lower overall viral load compared to Ae. aegypti, (2) the identification of multiple unknown phage contigs, and (3) the difference in competence for important human pathogens. How these viruses, phages, and bacteria influence the physiology and vector competence of mosquito hosts warrants further research. Electronic supplementary material The online version of this article (10.1186/s40168-019-0734-2) contains supplementary material, which is available to authorized users.
- Subjects :
- Microbiology (medical)
Viral metagenomics
viruses
Aedes aegypti
Genome, Viral
Mosquito Vectors
Microbiology
Genome
lcsh:Microbial ecology
03 medical and health sciences
Aedes
parasitic diseases
Animals
Human virome
Guadeloupe
Phylogeny
030304 developmental biology
Genetics
0303 health sciences
biology
030306 microbiology
Research
fungi
Culex quinquefasciatus
Single mosquito
biology.organism_classification
3. Good health
Culex
Phageome
Metagenomics
Viruses
[SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology
lcsh:QR100-130
Metagenome
Wolbachia
Core virome
Eukaryotic virome
Viral load
Subjects
Details
- Language :
- English
- ISSN :
- 20492618
- Database :
- OpenAIRE
- Journal :
- Microbiome, Microbiome, BioMed Central, 2019, 7, pp.121. ⟨10.1186/s40168-019-0734-2⟩, Microbiome, 2019, 7, pp.121. ⟨10.1186/s40168-019-0734-2⟩, Microbiome, Vol 7, Iss 1, Pp 1-20 (2019)
- Accession number :
- edsair.doi.dedup.....ca0cdfe69ae8bb103309d3cd487b9d37