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Viral Fitness Landscapes in Diverse Host Species Reveal Multiple Evolutionary Lines for the NS1 Gene of Influenza A Viruses
- Source :
- Cell reports, Cell Reports, Vol 29, Iss 12, Pp 3997-4009.e5 (2019)
- Publication Year :
- 2019
- Publisher :
- Elsevier BV, 2019.
-
Abstract
- SUMMARY Influenza A viruses (IAVs) have a remarkable tropism in their ability to circulate in both mammalian and avian species. The IAV NS1 protein is a multifunctional virulence factor that inhibits the type I interferon host response through a myriad of mechanisms. How NS1 has evolved to enable this remarkable property across species and its specific impact in the overall replication, pathogenicity, and host preference remain unknown. Here we analyze the NS1 evolutionary landscape and host tropism using a barcoded library of recombinant IAVs. Results show a surprisingly great variety of NS1 phenotypes according to their ability to replicate in different hosts. The IAV NS1 genes appear to have taken diverse and random evolutionary pathways within their multiple phylogenetic lineages. In summary, the high evolutionary plasticity of this viral protein underscores the ability of IAVs to adapt to multiple hosts and aids in our understanding of its global prevalence.<br />Graphical Abstract<br />In Brief Muñoz-Moreno et al. report that influenza A virus NS1 undergoes diverse and unpredictable evolutionary pathways based on its different phylogenetic lineages. A high-throughput approach using a barcoded library is used to test the interactions between NS1-recombinant viruses and to study their preference for specific or multiple hosts.
- Subjects :
- 0301 basic medicine
Viral protein
viruses
Evolutionary landscape
Host tropism
Viral Nonstructural Proteins
Biology
Virus Replication
medicine.disease_cause
Host Specificity
Article
General Biochemistry, Genetics and Molecular Biology
Madin Darby Canine Kidney Cells
Mice
03 medical and health sciences
Dogs
0302 clinical medicine
Orthomyxoviridae Infections
Interferon
medicine
Animals
lcsh:QH301-705.5
Gene
Phylogeny
Tropism
030304 developmental biology
Genetics
0303 health sciences
Phylogenetic tree
030306 microbiology
Host (biology)
virus diseases
Phenotype
Immunity, Innate
3. Good health
030104 developmental biology
lcsh:Biology (General)
Influenza A virus
Evolutionary biology
Host-Pathogen Interactions
Mutation
Female
030217 neurology & neurosurgery
medicine.drug
Subjects
Details
- ISSN :
- 22111247
- Volume :
- 29
- Database :
- OpenAIRE
- Journal :
- Cell Reports
- Accession number :
- edsair.doi.dedup.....ca074685236fa07d6fc6805946a55392