Back to Search
Start Over
miR‑638 suppresses proliferation by negatively regulating high mobility group A1 in ovarian cancer cells
- Source :
- Experimental and Therapeutic Medicine
- Publication Year :
- 2021
- Publisher :
- Spandidos Publications, 2021.
-
Abstract
- Ovarian cancer is one of the most common gynecological diseases with high mortality rates. Previous studies have shown that microRNA (miR)-638 is associated with tumorigenesis. The present study aimed to assess the role and underlying mechanisms of miR-638 in ovarian cancer. miR-638 expression was detected in ovarian cancer tissues and miR-638 was overexpressed or knocked down in ovarian cancer OVCAR-3 and Caov-3 cells. The clinical results revealed that miR-638 expression was downregulated in ovarian cancer tissues compared with in adjacent normal tissues. miR-638 expression was also found to be relatively low in OVCAR-3 cells whilst being relatively high in Caov-3 cells among the five ovarian cancer cell lines tested. miR-638 overexpression inhibited cell viability, arrested the cell cycle at the G1 phase and promoted apoptosis in OVCAR-3 cells. By contrast, miR-638 knockdown increased Caov-3 cell viability, facilitated cell cycle progression and inhibited apoptosis. miR-638 reduced the expression of high mobility group A1 (HMGA1) by directly targeting its 3' untranslated region. HMGA1 overexpression reversed the inhibition of proliferation induced by miR-638 overexpression in OVCAR-3 cells. These results suggest that miR-638 may serve to be a suppressor of ovarian cancer by regulating HMGA1, which may provide a potential therapeutic target for ovarian cancer.
- Subjects :
- Cancer Research
endocrine system diseases
Oncogene
proliferation
high mobility group A1
Cell
apoptosis
Cancer
Articles
General Medicine
Cell cycle
Biology
medicine.disease_cause
medicine.disease
female genital diseases and pregnancy complications
microRNA-638
ovarian cancer
medicine.anatomical_structure
Immunology and Microbiology (miscellaneous)
microRNA
medicine
Cancer research
Viability assay
Carcinogenesis
Ovarian cancer
Subjects
Details
- ISSN :
- 17921015 and 17920981
- Volume :
- 22
- Database :
- OpenAIRE
- Journal :
- Experimental and Therapeutic Medicine
- Accession number :
- edsair.doi.dedup.....c9f7268657b0e92d1f665143d8c07d02