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Regulation of kinesin-1 activity by the Salmonella enterica effectors PipB2 and SifA
- Source :
- Journal of Cell Science, Journal of Cell Science, Company of Biologists, 2020, 133 (9), pp.jcs239863. ⟨10.1242/jcs.239863⟩
- Publication Year :
- 2020
- Publisher :
- The Company of Biologists, 2020.
-
Abstract
- Salmonella enterica is an intracellular bacterial pathogen. The formation of its replication niche, which is composed of a vacuole associated with a network of membrane tubules, depends on the secretion of a set of bacterial effector proteins whose activities deeply modify the functions of the eukaryotic host cell. By recruiting and regulating the activity of the kinesin-1 molecular motor, Salmonella effectors PipB2 and SifA play an essential role in the formation of the bacterial compartments. In particular, they allow the formation of tubules from the vacuole and their extension along the microtubule cytoskeleton, and thus promote membrane exchanges and nutrient supply. We have developed in vitro and in cellulo assays to better understand the specific role played by these two effectors in the recruitment and regulation of kinesin-1. Our results reveal a specific interaction between the two effectors and indicate that, contrary to what studies on infected cells suggested, interaction with PipB2 is sufficient to relieve the autoinhibition of kinesin-1. Finally, they suggest the involvement of other Salmonella effectors in the control of the activity of this molecular motor. This article has an associated First Person interview with the first author of the paper.
- Subjects :
- Salmonella
[SDV]Life Sciences [q-bio]
macromolecular substances
[SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC]
[SDV.BC]Life Sciences [q-bio]/Cellular Biology
Vacuole
medicine.disease_cause
03 medical and health sciences
0302 clinical medicine
[SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases
[SDV.BC.IC]Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB]
Molecular motor
medicine
Secretion
ComputingMilieux_MISCELLANEOUS
030304 developmental biology
0303 health sciences
biology
Effector
[SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology
Cell Biology
biology.organism_classification
[SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biomolecules [q-bio.BM]
Cell biology
[SDV.BBM.BP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biophysics
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology
Salmonella enterica
[SDV.IMM]Life Sciences [q-bio]/Immunology
Kinesin
[PHYS.COND.CM-SCM]Physics [physics]/Condensed Matter [cond-mat]/Soft Condensed Matter [cond-mat.soft]
030217 neurology & neurosurgery
Intracellular
Subjects
Details
- ISSN :
- 14779137 and 00219533
- Volume :
- 133
- Database :
- OpenAIRE
- Journal :
- Journal of Cell Science
- Accession number :
- edsair.doi.dedup.....c9e5885f9ba80c201bc470c69b86df32