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Application of DNA filter hybridization and PCR to distinguish between human and non-human tissues of poor quality
- Source :
- International Journal of Legal Medicine. 105:307-309
- Publication Year :
- 1993
- Publisher :
- Springer Science and Business Media LLC, 1993.
-
Abstract
- The present report describes a novel approach for the identification of human or non-human specimens after long-term storage in a badly preserved state. The application of the PCR-technique (polymerase chain reaction) using human-specific primers as well as Southern blot (filter) hybridization of the sample DNA to a primate-specific DNA probe enabled us to extend the positive identification beyond the limits of conventional methods such as serological or morphological examinations.
- Subjects :
- Swine
Hybridization probe
Nucleic Acid Hybridization
Biology
Polymerase Chain Reaction
Molecular biology
Pathology and Forensic Medicine
law.invention
Blot
Blotting, Southern
Nucleic acid thermodynamics
Dogs
Real-time polymerase chain reaction
Species Specificity
law
Postmortem Changes
Multiplex polymerase chain reaction
Allele-specific oligonucleotide
Animals
Humans
DNA Probes
Homicide
Polymerase chain reaction
Southern blot
Subjects
Details
- ISSN :
- 14371596 and 09379827
- Volume :
- 105
- Database :
- OpenAIRE
- Journal :
- International Journal of Legal Medicine
- Accession number :
- edsair.doi.dedup.....c9b71fc4c5a12ba7a7469f2f785d3545
- Full Text :
- https://doi.org/10.1007/bf01222112