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Application of DNA filter hybridization and PCR to distinguish between human and non-human tissues of poor quality

Authors :
Hans-Joachim Wagner
Roman Müllenbach
Nikolaus Blin
Darius Makuch
Source :
International Journal of Legal Medicine. 105:307-309
Publication Year :
1993
Publisher :
Springer Science and Business Media LLC, 1993.

Abstract

The present report describes a novel approach for the identification of human or non-human specimens after long-term storage in a badly preserved state. The application of the PCR-technique (polymerase chain reaction) using human-specific primers as well as Southern blot (filter) hybridization of the sample DNA to a primate-specific DNA probe enabled us to extend the positive identification beyond the limits of conventional methods such as serological or morphological examinations.

Details

ISSN :
14371596 and 09379827
Volume :
105
Database :
OpenAIRE
Journal :
International Journal of Legal Medicine
Accession number :
edsair.doi.dedup.....c9b71fc4c5a12ba7a7469f2f785d3545
Full Text :
https://doi.org/10.1007/bf01222112