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Sodium thiosulfate-supplemented UW solution protects renal grafts against prolonged cold ischemia-reperfusion injury in a murine model of syngeneic kidney transplantation
- Source :
- Biomedicine & Pharmacotherapy, Vol 145, Iss, Pp 112435-(2022)
- Publication Year :
- 2022
- Publisher :
- Elsevier, 2022.
-
Abstract
- Introduction: Cold ischemia-reperfusion injury (IRI) is an inevitable event that increases post-transplant complications. We have previously demonstrated that supplementation of University of Wisconsin (UW) solution with non-FDA-approved hydrogen sulfide (H2S) donor molecules minimizes cold IRI and improves renal graft function after transplantation. The present study investigates whether an FDA-approved H2S donor molecule, sodium thiosulfate (STS), will have the same or superior effect in a clinically relevant rat model of syngeneic orthotopic kidney transplantation. Method: Thirty Lewis rats underwent bilateral nephrectomy followed by syngeneic orthotopic transplantation of the left kidney after 24-hour preservation in either UW or UW+STS solution at 4 °C. Rats were monitored to post-transplant day 14 and sacrificed to assess renal function (urine output, serum creatinine and blood urea nitrogen). Kidney sections were stained with H&E, TUNEL, CD68, and myeloperoxidase (MPO) to detect acute tubular necrosis (ATN), apoptosis, macrophage infiltration, and neutrophil infiltration. Result: UW+STS grafts showed significantly improved graft function immediately after transplantation, with improved recipient survival compared to UW grafts (p
- Subjects :
- Male
Ischemia-reperfusion injury (IRI)
medicine.medical_specialty
Static cold storage (SCS)
Adenosine
Allopurinol
Organ Preservation Solutions
Urology
Thiosulfates
Renal function
Apoptosis
RM1-950
Kidney Function Tests
Blood Urea Nitrogen
Kidney transplantation
Raffinose
Sodium thiosulfate (STS)
medicine
Animals
Insulin
Viaspan
Blood urea nitrogen
Acute tubular necrosis
Pharmacology
Kidney
business.industry
Cold Ischemia
Graft and recipient survival
General Medicine
medicine.disease
Glutathione
Rats
Transplantation
Survival Rate
medicine.anatomical_structure
Rats, Inbred Lew
Creatinine
Reperfusion Injury
Therapeutics. Pharmacology
business
Reperfusion injury
Subjects
Details
- Language :
- English
- ISSN :
- 07533322
- Volume :
- 145
- Database :
- OpenAIRE
- Journal :
- Biomedicine & Pharmacotherapy
- Accession number :
- edsair.doi.dedup.....c9af77ecd3c0b5c5b7ddfda05f9cfc3a