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The method of purifying bioengineered spider silk determines the silk sphere properties
- Source :
- Scientific Reports
- Publication Year :
- 2016
- Publisher :
- Nature Publishing Group, 2016.
-
Abstract
- Bioengineered spider silks are a biomaterial with great potential for applications in biomedicine. They are biocompatible,biodegradable and can self-assemble into films, hydrogels, scaffolds, fibers, capsules and spheres. A novel, tag-free, bioengineered spider silk named MS2(9x) was constructed. It is a 9-mer of the consensus motif derived from MaSp2–the spidroin of Nephila clavipes dragline silk. Thermal and acidic extraction methods were used to purify MS2(9x). Both purification protocols gave a similar quantity and quality of soluble silk; however, they differed in the secondary structure and zeta potential value. Spheres made of these purified variants differed with regard to critical features such as particle size, morphology, zeta potential and drug loading. Independent of the purification method, neither variant of the MS2(9x) spheres was cytotoxic, which confirmed that both methods can be used for biomedical applications. However, this study highlights the impact that the applied purification method has on the further biomaterial properties.
- Subjects :
- Liquid-Liquid Extraction
Biomedical Engineering
Silk
Biocompatible Materials
02 engineering and technology
010402 general chemistry
01 natural sciences
Protein Structure, Secondary
Article
Spectroscopy, Fourier Transform Infrared
Zeta potential
Animals
Spider silk
Amino Acid Sequence
Purification methods
Drug Carriers
Multidisciplinary
biology
Polymer science
Chemistry
Spidroin
Nephila clavipes
fungi
Biomaterial
Spiders
021001 nanoscience & nanotechnology
biology.organism_classification
equipment and supplies
Recombinant Proteins
0104 chemical sciences
SILK
Self-healing hydrogels
Microscopy, Electron, Scanning
0210 nano-technology
Subjects
Details
- Language :
- English
- ISSN :
- 20452322
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....c9a72c2fe0c43e7202d5ee4a4e878cf6
- Full Text :
- https://doi.org/10.1038/srep28106