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Histochemical and Immunological Analyses of Differentiating Skeletal Muscle Fibers of the Postnatal Rat
- Source :
- Cells Tissues Organs. 143:1-6
- Publication Year :
- 1992
- Publisher :
- S. Karger AG, 1992.
-
Abstract
- Fiber type differentiation as revealed by myosin ATPase activities and the immunological analysis of myosin light chains (MLC) by the Western blotting method were done on the postnatal rat soleus and extensor digitorum longus (EDL) muscles. In the soleus, type 1 (slow-twitch) muscle fibers gradually increase in percentage after postnatal day 10, and, in the EDL, the differentiation of type 2A and 2B (fast-twitch) muscle fibers is also pronounced between postnatal days 10 and 15. The present immunological analysis of MLC indicates that the antigenicity of LclS becomes more intense with a rapid decrease in anti-genicity of Lc2F from postnatal day 7 in the soleus, while the antigenicity of LclF becomes noticeable during the same period in the EDL. These results suggest that the differentiation of slow-twitch fibers is mainly due to that of Lc1S in the soleus and the differentiation of fast-twitch fibers is mainly due to that of Lc1F in the EDL
- Subjects :
- medicine.medical_specialty
animal structures
Histology
Myosin light-chain kinase
Myosin ATPase
Cellular differentiation
Immunoblotting
Myosins
Muscle Development
Antibodies
Extensor digitorum muscle
Internal medicine
Myosin
medicine
Animals
Soleus muscle
biology
Histocytochemistry
Muscles
musculoskeletal, neural, and ocular physiology
Age Factors
Cell Differentiation
Rats, Inbred Strains
musculoskeletal system
Rats
Cell biology
Blot
Endocrinology
biology.protein
Anatomy
Antibody
tissues
Subjects
Details
- ISSN :
- 14226421 and 14226405
- Volume :
- 143
- Database :
- OpenAIRE
- Journal :
- Cells Tissues Organs
- Accession number :
- edsair.doi.dedup.....c9a339b9a64c9d72c0a87a6849afface