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Using primary murine intestinal enteroids to study dietary TAG absorption, lipoprotein synthesis, and the role of apoC-III in the intestine[S]
- Source :
- Journal of Lipid Research, Vol 58, Iss 5, Pp 853-865 (2017)
- Publication Year :
- 2017
- Publisher :
- The American Society for Biochemistry and Molecular Biology, 2017.
-
Abstract
- Since its initial report in 2009, the intestinal enteroid culture system has been a powerful tool used to study stem cell biology and development in the gastrointestinal tract. However, a major question is whether enteroids retain intestinal function and physiology. There have been significant contributions describing ion transport physiology of human intestinal organoid cultures, as well as physiology of gastric organoids, but critical studies on dietary fat absorption and chylomicron synthesis in primary intestinal enteroids have not been undertaken. Here we report that primary murine enteroid cultures recapitulate in vivo intestinal lipoprotein synthesis and secretion, and reflect key aspects of the physiology of intact intestine in regard to dietary fat absorption. We also show that enteroids can be used to elucidate intestinal mechanisms behind CVD risk factors, including tissue-specific apolipoprotein functions. Using enteroids, we show that intestinal apoC-III overexpression results in the secretion of smaller, less dense chylomicron particles along with reduced triacylglycerol secretion from the intestine. This model significantly expands our ability to test how specific genes or genetic polymorphisms function in dietary fat absorption and the precise intestinal mechanisms that are critical in the etiology of metabolic disease.
- Subjects :
- dietary fat absorption
0301 basic medicine
medicine.medical_specialty
Apolipoprotein B
Mice, Transgenic
QD415-436
Absorption (skin)
Biology
Biochemistry
03 medical and health sciences
Mice
Endocrinology
stem cells
In vivo
Internal medicine
Chylomicrons
Organoid
medicine
Animals
Humans
Secretion
Intestinal Mucosa
organoids
Research Articles
Triglycerides
Gastrointestinal tract
Apolipoprotein C-III
Cell Differentiation
Cell Biology
Dietary Fats
Intestines
Mice, Inbred C57BL
030104 developmental biology
Absorption, Physicochemical
Organ Specificity
intestinal lipid transport
biology.protein
Function (biology)
Chylomicron
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Journal of Lipid Research, Vol 58, Iss 5, Pp 853-865 (2017)
- Accession number :
- edsair.doi.dedup.....c997fe4733bdb67adc40c5d8b5d1ef85