Back to Search
Start Over
Transduction, restriction and recombination patterns in Escherichia coli
- Source :
- Genetics. 139:35-43
- Publication Year :
- 1995
- Publisher :
- Oxford University Press (OUP), 1995.
-
Abstract
- Chromosomal DNA from several Escherichia coli reference (ECOR) strains was transduced by bacteriophage P1 into E. coli strain K12 W3110 trpA33. Recombination patterns of the transductants were determined by restriction fragment length polymorphism over a 40-kb region centering on a single marker (trpA+) in the tryptophan operon. These experiments demonstrate that transduction between different strains of E. coli can result in recombinational replacements that are small in comparison to the entrant molecule (replacements average 8-14 kb, whereas P1 packages approximately 100 kb) often in a series of discrete segments. The transduction patterns generated resemble the natural mosaic sequence patterns of the ECOR strains described in previous work. Extensive polymorphisms in the restriction-modification systems of the ECOR strains are a possible explanation for the sequence patterns in nature. To test this possibility two transductants were back-transduced into strain K12 W3110 trpA33. The resulting patterns were strikingly different from the original transductions. The size of the replacements was greater, and no multiple replacements were observed, suggesting a role for restriction-modification systems in the transduction patterns and perhaps for the mosaic sequence patterns in nature.
- Subjects :
- Recombination, Genetic
Genetics
Sequence analysis
Sequence Analysis, DNA
Viral Plaque Assay
Investigations
Biology
medicine.disease_cause
DNA Restriction-Modification Enzymes
Molecular biology
trp operon
Transduction (genetics)
chemistry.chemical_compound
chemistry
Transduction, Genetic
Escherichia coli
medicine
Bacteriophage P1
Restriction fragment length polymorphism
Polymorphism, Restriction Fragment Length
Recombination
DNA
Subjects
Details
- ISSN :
- 19432631
- Volume :
- 139
- Database :
- OpenAIRE
- Journal :
- Genetics
- Accession number :
- edsair.doi.dedup.....c98c459cc6064a1839b4a59cc04ab896