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Wide distribution of Plasmodium ovale in Myanmar
- Source :
- Tropical Medicine and International Health. 7:231-239
- Publication Year :
- 2002
- Publisher :
- Wiley, 2002.
-
Abstract
- The presence of Plasmodium ovale has never been previously reported in Myanmar. Using blood samples obtained in many villages across the country between 1996 and 2000, molecular diagnosis of Plasmodium species was made with semi- or full-nested polymerase chain reaction (PCR) with species-specific primers, followed by agarose gel electrophoresis to detect amplification products. The presence of P. ovale was also confirmed with the another PCR-based diagnosis, the microtiterplate hybridization (MPH) method using species-specific probes. Both methods target the A type of the small subunit ribosomal RNA gene of the four human malaria parasites. Plasmodium ovale DNA was amplified in samples from 65 (4.9%) of 1323 PCR-positive patients, with perfect agreement between results obtained by nested PCR and MPH. Only four P. ovale-infected patients had single-species infection; all others were coinfected with P. falciparum, P. vivax and/or P. malariae. Quadruple infections were observed in six subjects. Parasites with typical P. ovale morphology were found in only 19 patients by conventional microscopy of Giemsa-stained thin smears or fluorescence microscopy of acridine orange-stained thin smears. Plasmodium ovale infections were found in villages situated in the southern, central and western regions of Myanmar, suggesting that P. ovale may be widely distributed in this country.
- Subjects :
- Adult
Male
Plasmodium
Adolescent
Myanmar
Polymerase Chain Reaction
law.invention
Apicomplexa
Species Specificity
law
parasitic diseases
medicine
Animals
Humans
Child
Polymerase chain reaction
Aged
biology
Public Health, Environmental and Occupational Health
DNA, Protozoan
Middle Aged
Plasmodium ovale
biology.organism_classification
medicine.disease
Virology
Malaria
Infectious Diseases
Child, Preschool
Agarose gel electrophoresis
Protozoa
Female
Parasitology
Nested polymerase chain reaction
Subjects
Details
- ISSN :
- 13653156 and 13602276
- Volume :
- 7
- Database :
- OpenAIRE
- Journal :
- Tropical Medicine and International Health
- Accession number :
- edsair.doi.dedup.....c979c467624b466b761eca47c2cc03b7