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Plasma-activated water: a new and effective alternative for duodenoscope reprocessing
- Source :
- Infection and Drug Resistance
-
Abstract
- Gheorghe G BÄlan,1 Irina RoÅca,2 Elena-Laura Ursu,2 Florica Doroftei,2 Andra-Cristina BostÄnaru,3 Eugen Hnatiuc,4 Valentin NÄstasÄ,3 Vasile Åandru,5 Gabriela ÅtefÄnescu,1 Anca Trifan,1 Mihai MareÅ3 1Grigore T. Popa University of Medicine and Pharmacy, IaÅi, Romania; 2“Petru Poni” Institute of Macromolecular Chemistry, IaÅi, Romania; 3Ion Ionescu de la Brad University of Agricultural Sciences and Veterinary Medicine of Iasi, IaÅi, Romania; 4“Gheorghe Asachi” Technical University, IaÅi, Romania; 5Department of Gastroenterology Research, Clinical Emergency Hospital of Bucharest, Bucharest, Romania Introduction: Duodenoscopes have been widely used for both diagnostic and therapeutic endoscopic retrograde cholangiopancreatography procedures. Numerous outbreaks of duodenoscope-associated infections involving multidrug-resistant bacteria have recently been reported. Plasma activated water (PAW) has been widely considered an effective agent for surface decontamination and is increasingly used for disinfection of medical equipment. The aim of this study was to evaluate whether the duodenoscopes currently on market are suited for the repeated use of PAW and to test the efficacy of PAW for their disinfection. Materials and methods: In order to evaluate the disinfection efficacy and the required time of contact, the duodenoscope samples were contaminated by immersing them in fasted-state simulated intestinal fluid containing Escherichia coli, Klebsiella pneumoniae, Acinetobacter baumannii, and Pseudomonas aeruginosa, prior to PAW exposure. In order to test the duodenoscope polymer compatibility with PAW, a challenge test was conducted by immersing the samples in PAW for 30 minutes daily for 45 consecutive days. Results: Significant reductions in bacterial populations were achieved after 30 minutes of PAW treatment, indicating a high-level disinfection. Atomic force microscopy and scanning electron microscopy were used to demonstrate that repeated PAW treatment of duodenoscope coating polymer samples did not result in significant differences in morphological surface between the treated and untreated samples. Energy-dispersive X-ray spectroscopy analysis also showed no significant differences between the elemental composition of the duodenoscope coating polymer samples before and after repeated PAW treatment. Conclusion: Considering these preliminary results, PAW could be considered as a new alternative for duodenoscope reprocessing. Keywords: PAW, antibacterial activity, duodenoscope, disinfection
- Subjects :
- Pharmacology
Elemental composition
biology
Atomic force microscopy
Chemistry
Duodenoscopes
biology.organism_classification
Intestinal fluid
3. Good health
Acinetobacter baumannii
03 medical and health sciences
0302 clinical medicine
Infectious Diseases
antibacterial activity
Infection and Drug Resistance
030211 gastroenterology & hepatology
Pharmacology (medical)
PAW
030212 general & internal medicine
duodenoscope
disinfection
Biomedical engineering
Original Research
Subjects
Details
- Language :
- English
- ISSN :
- 11786973
- Volume :
- 11
- Database :
- OpenAIRE
- Journal :
- Infection and Drug Resistance
- Accession number :
- edsair.doi.dedup.....c96c413d1f6e2cbe730a0e6644908c8d
- Full Text :
- https://doi.org/10.2147/idr.s159243