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Renal medullary gene expression in aquaporin-1 null mice
- Source :
- American Journal of Physiology-Renal Physiology. 288:F315-F321
- Publication Year :
- 2005
- Publisher :
- American Physiological Society, 2005.
-
Abstract
- Mice that lack the aquaporin-1 gene (AQP1) lack a functional countercurrent multiplier mechanism, fail to concentrate the inner medullary (IM) interstitium, and present with a urinary concentrating defect. In this study, we use DNA microarrays to identify the gene expression profile of the IM of AQP1 null mice and corresponding changes in gene expression resulting from a loss of a hypertonic medullary interstitium. An ANOVA analysis model, CARMA, was used to isolate the knockout effect while taking into account experimental variability associated with microarray studies. In this study 5,701 genes of the possible ∼12,000 genes on the array were included in the ANOVA; 531 genes were identified as demonstrating a >1.5-fold up- or downregulation between the wild-type and knockout groups. We randomly selected 35 genes for confirmation by real-time PCR, and 29 of the 35 genes were confirmed using this method. The overall pattern of gene expression in the AQP1 null mice was one of downregulation compared with gene expression in the renal medullas of the wild-type mice. Heat shock proteins 105 and 94, aldose reductase, adenylate kinase 2, aldolase B, aldehyde reductase 6, and p8 were decreased in the AQP1 null mice. Carboxylesterase 3, matrilin 2, lipocalin 2, and transforming growth factor-α were increased in IM of AQP1 null mice. In addition, we observed a loss of vasopressin type 2 receptor mRNA expression in renal medullas of the AQP1 null mice. Thus the loss of the hyperosmotic renal interstitium, due to a loss of the concentrating mechanism, drastically altered not only the phenotype of these animals but also their renal medullary gene expression profile.
- Subjects :
- medicine.medical_specialty
Medullary cavity
Vasopressins
Physiology
Urinary system
Countercurrent multiplication
Aquaporin
Biology
Aquaporins
Polymerase Chain Reaction
Mice
Internal medicine
Gene expression
medicine
Animals
Oligonucleotide Array Sequence Analysis
Mice, Knockout
Regulation of gene expression
Kidney Medulla
Aquaporin 1
Gene Expression Profiling
Cell biology
Gene expression profiling
Phenotype
Endocrinology
Gene Expression Regulation
Blood Group Antigens
Subjects
Details
- ISSN :
- 15221466 and 1931857X
- Volume :
- 288
- Database :
- OpenAIRE
- Journal :
- American Journal of Physiology-Renal Physiology
- Accession number :
- edsair.doi.dedup.....c962972ade3b4d74d41580bee20c6e31
- Full Text :
- https://doi.org/10.1152/ajprenal.00207.2004