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Functional characterization of Autographa californica multiple nucleopolyhedrovirus gp16 (ac130)
- Source :
- Virology. :341-352
- Publication Year :
- 2014
- Publisher :
- Elsevier BV, 2014.
-
Abstract
- To investigate the function of Autographa californica multiple nucleopolyhedrovirus (AcMNPV) gp16, multiple gp16-knockout and repair mutants were constructed and characterized. No obvious difference in productivity of budded virus, DNA synthesis, late gene expression and morphogenesis was observed between gp16-knockout and repair viruses, but gp16 deletion resulted in six hours of lengthening in ST50 to the third instar Spodoptera exigua larvae in bioassays. GP16 was fractionated mainly in the light membrane fraction, by subcellular fractionation. A GP16-EGFP fusion protein was predominantly localized close around the nuclear membrane in infected cells, being coincident with formation of the vesicles associated with the nuclear membrane, which hosted nucleocapsids released from the nucleus. These data suggest that gp16 is not required for viral replication, but may be involved in membrane trafficking associated with the envelopment/de-envelopment of budded viruses when they cross over the nuclear membrane and pass through cytoplasm.
- Subjects :
- Nuclear Envelope
viruses
Mutant
Spodoptera
Virus Replication
Viral Proteins
gp16
Virology
medicine
Animals
Nuclear membrane
biology
Virus Assembly
fungi
biology.organism_classification
Fusion protein
Molecular biology
Nucleopolyhedroviruses
Autographa californica
ac130
Protein Transport
medicine.anatomical_structure
Viral replication
Cytoplasm
Autographa californica multiple nucleopolyhedrovirus
gp16 knockout
Cell fractionation
Subjects
Details
- ISSN :
- 00426822
- Database :
- OpenAIRE
- Journal :
- Virology
- Accession number :
- edsair.doi.dedup.....c94ff1fa80dbbae78a48f5ec5b2f06ef
- Full Text :
- https://doi.org/10.1016/j.virol.2014.07.030