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Remodeling the Integration of Lipid Metabolism Between Liver and Adipose Tissue by Dietary Methionine Restriction in Rats
- Source :
- Diabetes
- Publication Year :
- 2013
- Publisher :
- American Diabetes Association, 2013.
-
Abstract
- Dietary methionine restriction (MR) produces an integrated series of biochemical and physiological responses that improve biomarkers of metabolic health, limit fat accretion, and enhance insulin sensitivity. Using transcriptional profiling to guide tissue-specific evaluations of molecular responses to MR, we report that liver and adipose tissue are the primary targets of a transcriptional program that remodeled lipid metabolism in each tissue. The MR diet produced a coordinated downregulation of lipogenic genes in the liver, resulting in a corresponding reduction in the capacity of the liver to synthesize and export lipid. In contrast, the transcriptional response in white adipose tissue (WAT) involved a depot-specific induction of lipogenic and oxidative genes and a commensurate increase in capacity to synthesize and oxidize fatty acids. These responses were accompanied by a significant change in adipocyte morphology, with the MR diet reducing cell size and increasing mitochondrial density across all depots. The coordinated transcriptional remodeling of lipid metabolism between liver and WAT by dietary MR produced an overall reduction in circulating and tissue lipids and provides a potential mechanism for the increase in metabolic flexibility and enhanced insulin sensitivity produced by the diet.
- Subjects :
- Male
medicine.medical_specialty
Adipose Tissue, White
Endocrinology, Diabetes and Metabolism
Blotting, Western
Down-Regulation
Gene Expression
Adipose tissue
030209 endocrinology & metabolism
White adipose tissue
Mitochondrion
Biology
Real-Time Polymerase Chain Reaction
Eating
03 medical and health sciences
chemistry.chemical_compound
Methionine
0302 clinical medicine
Insulin resistance
Downregulation and upregulation
Leucine
Internal medicine
Adipocyte
Internal Medicine
medicine
Animals
Original Research
030304 developmental biology
2. Zero hunger
0303 health sciences
Fatty Acids
Lipid metabolism
Lipid Metabolism
medicine.disease
Rats, Inbred F344
Diet
Mitochondria
Rats
Metabolism
Endocrinology
Liver
chemistry
Insulin Resistance
Energy Metabolism
Biomarkers
Subjects
Details
- ISSN :
- 1939327X and 00121797
- Volume :
- 62
- Database :
- OpenAIRE
- Journal :
- Diabetes
- Accession number :
- edsair.doi.dedup.....c94bf6ca9050985065dceb3cdb3f2159
- Full Text :
- https://doi.org/10.2337/db13-0501