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Biosynthesis of uridine diphosphate N-acetyl-D-mannosaminuronic acid from uridine diphosphate N-acetyl-D-glucosamine in Escherichia coli: Separation of enzymes responsible for epimerization and dehydrogenation
- Source :
- Biochemical and Biophysical Research Communications. 66:1506-1512
- Publication Year :
- 1975
- Publisher :
- Elsevier BV, 1975.
-
Abstract
- The biosynthesis of uridine diphosphate N-acetyl-D-mannosaminuronic acid from uridine diphosphate N-acetyl-D-glucosamine occurs in two steps. The enzyme responsible for the first step, the epimerization of uridine diphosphate N-acetyl-D-glucosamine to uridine diphosphate N-acetyl-D-mannosamine, is separated by means of hydroxylapatite chromatography from the enzyme for the second step, the NAD-linked dehydrogenation of uridine diphosphate N-acetyl-D-mannosamine. At equilibrium of the epimerase reaction, the ratio of the glucosamine residue to the mannosamine residue is about 9:1.
- Subjects :
- Biophysics
Biochemistry
chemistry.chemical_compound
Residue (chemistry)
Biosynthesis
Glucosamine
Escherichia coli
Molecular Biology
Nucleotide salvage
chemistry.chemical_classification
Uridine Diphosphate N-Acetylglucosamine
Chemistry
Hexuronic Acids
Hexosamines
Mannosamine
Cell Biology
Uridine Diphosphate Sugars
Alcohol Oxidoreductases
Kinetics
Uridine diphosphate
Uronic Acids
Enzyme
Epimer
Carbohydrate Epimerases
Mannose
Subjects
Details
- ISSN :
- 0006291X
- Volume :
- 66
- Database :
- OpenAIRE
- Journal :
- Biochemical and Biophysical Research Communications
- Accession number :
- edsair.doi.dedup.....c91db50c29dbd8223e0300a5aca06d96