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Decoding neuronal composition and ontogeny of individual hypothalamic nuclei
Decoding neuronal composition and ontogeny of individual hypothalamic nuclei
- Source :
- Neuron
- Publication Year :
- 2020
-
Abstract
- The hypothalamus plays crucial roles in regulating endocrine, autonomic, and behavioral functions via its diverse nuclei and neuronal subtypes. The developmental mechanisms underlying ontogenetic establishment of different hypothalamic nuclei and generation of neuronal diversity remain largely unknown. Here, we show that combinatorial T-box 3 (TBX3), orthopedia homeobox (OTP), and distal-less homeobox (DLX) expression delineates all arcuate nucleus (Arc) neurons and defines four distinct subpopulations, whereas combinatorial NKX2.1/SF1 and OTP/DLX expression identifies ventromedial hypothalamus (VMH) and tuberal nucleus (TuN) neuronal subpopulations, respectively. Developmental analysis indicates that all four Arc subpopulations are mosaically and simultaneously generated from embryonic Arc progenitors, whereas glutamatergic VMH neurons and GABAergic TuN neurons are sequentially generated from common embryonic VMH progenitors. Moreover, clonal lineage-tracing analysis reveals that diverse lineages from multipotent radial glia progenitors orchestrate Arc and VMH-TuN establishment. Together, our study reveals cellular mechanisms underlying generation and organization of diverse neuronal subtypes and ontogenetic establishment of individual nuclei in the mammalian hypothalamus.
- Subjects :
- 0301 basic medicine
Hypothalamus
Glutamic Acid
Nerve Tissue Proteins
Biology
Article
Animals, Genetically Modified
03 medical and health sciences
Glutamatergic
Mice
0302 clinical medicine
Arcuate nucleus
medicine
Animals
Cell Lineage
gamma-Aminobutyric Acid
Homeodomain Proteins
Neurons
Arc (protein)
General Neuroscience
Stem Cells
Arcuate Nucleus of Hypothalamus
Embryonic stem cell
Mice, Inbred C57BL
030104 developmental biology
medicine.anatomical_structure
nervous system
Ventromedial Hypothalamic Nucleus
GABAergic
Homeobox
T-Box Domain Proteins
Neuroscience
Nucleus
Neuroglia
030217 neurology & neurosurgery
Transcription Factors
Subjects
Details
- ISSN :
- 10974199
- Volume :
- 109
- Issue :
- 7
- Database :
- OpenAIRE
- Journal :
- Neuron
- Accession number :
- edsair.doi.dedup.....c913555554752709769d46d6ea1f6a16