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Screening for cyanobacterial toxins in bloom and strain samples by thin layer chromatography
- Source :
- University of Helsinki
- Publication Year :
- 1996
- Publisher :
- Elsevier BV, 1996.
-
Abstract
- Two thin layer chromatographic (TLC) procedures were evaluated for the capacity screening of cyanobacterial peptide hepatotoxins and anatoxin-a in laboratory-cultured strain samples and in natural bloom samples. Seventy-five samples previously investigated with the reference methods, the chief of which was mouse bioassay, were analyzed blindly by TLC. Forty-three of the samples were hepatotoxic according to the reference methods, and 39 of them were found to contain peptide hepatotoxins by TLC coupled to u.v.-densitometry. Seven samples contained anatoxin-a according to the reference methods, and five of these were found to contain anatoxin-a by TLC, using derivatization with Fast Black K salt. Four of the samples which were neurotoxic by mouse bioassay also contained hepatotoxins by TLC. Twenty-four samples were nontoxic according to the reference methods, and in 23 of these no toxins were found by TLC either.
- Subjects :
- chemistry.chemical_classification
Environmental Engineering
Chromatography
Ecological Modeling
Microcystin
respiratory system
Biology
biology.organism_classification
Pollution
Nodularin
High-performance liquid chromatography
humanities
Thin-layer chromatography
respiratory tract diseases
Anatoxin-a
chemistry.chemical_compound
chemistry
Microcystis
Bioassay
lipids (amino acids, peptides, and proteins)
Derivatization
Waste Management and Disposal
circulatory and respiratory physiology
Water Science and Technology
Civil and Structural Engineering
Subjects
Details
- ISSN :
- 00431354
- Volume :
- 30
- Database :
- OpenAIRE
- Journal :
- Water Research
- Accession number :
- edsair.doi.dedup.....c90ecda4f1e3ac012e23214cfeeeb533