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BoLA-6*01301 and BoLA-6*01302, two allelic variants of the A18 haplotype, present the same epitope from the Tp1 antigen of Theileria parva
- Source :
- Veterinary immunology and immunopathology. 167(1-2)
- Publication Year :
- 2015
-
Abstract
- We have recently shown that the BoLA-A18 variant haplotype (BoLA-6*01302) is more prevalent than the BoLA-A18 haplotype (BoLA-6*01301) in a sample of Holstein/Friesian cattle in Kenya. These MHC class I allelic variants differ by a single amino acid polymorphism (Glu97 to Leu97) in the peptide-binding groove. We have previously mapped an 11-mer peptide epitope from the Theileria parva antigen Tp1 (Tp1 214–224 ) that is presented by BoLA-6*01301. Crystal structure data indicates that Glu97 in the MHC molecule plays a role in epitope binding through electro-static interaction with a lysine residue in position 5 of the epitope, which also functions as an additional anchor residue. In contrast to expectations, we demonstrate that the amino acid substitution in BoLA-6*01302 does not divert the CTL response away from Tp1 214–224 . The two MHC molecules exhibit similar affinity for the Tp1 epitope and can present the epitope to parasite-specific CTLs derived from either BoLA allelic variants. These data confirm that this BoLA polymorphism does not alter Tp1 epitope specificity and that both allelic variants can be used for Tp1 vaccine studies.
- Subjects :
- Theileria parva
Immunology
Cytotoxic T cells
Antigens, Protozoan
Biology
Major histocompatibility complex
MHC polymorphism
Epitope
Cell Line
Epitopes
CTL epitope
Antigen
MHC class I
Cytotoxic T cell
Animals
BoLA
Amino Acid Sequence
Alleles
Genetics
Antigen Presentation
General Veterinary
Linear epitope
Haplotype
Histocompatibility Antigens Class I
biology.organism_classification
veterinary(all)
Molecular biology
Theileriasis
Amino Acid Substitution
Haplotypes
biology.protein
Cattle
T-Lymphocytes, Cytotoxic
Subjects
Details
- ISSN :
- 18732534
- Volume :
- 167
- Issue :
- 1-2
- Database :
- OpenAIRE
- Journal :
- Veterinary immunology and immunopathology
- Accession number :
- edsair.doi.dedup.....c8fcb2e44bed00ff8d71fbf1098d57d5