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Vertical Transmission of Zika Virus (Flaviviridae, Flavivirus) in Amazonian Aedes aegypti (Diptera: Culicidae) Delays Egg Hatching and Larval Development of Progeny
- Source :
- Journal of Medical Entomology, Repositório Institucional da UFOP, Universidade Federal de Ouro Preto (UFOP), instacron:UFOP
- Publication Year :
- 2019
- Publisher :
- Oxford University Press, 2019.
-
Abstract
- Zika virus (ZIKV) has emerged as a globally important arbovirus and has been reported from all states of Brazil. The virus is primarily transmitted to humans through the bite of an infective Aedes aegypti (Linnaeus, 1762) or Aedes albopictus (Skuse, 1895). However, it is important to know if ZIKV transmission also occurs from Ae. aegypti through infected eggs to her offspring. Therefore, a ZIKV and dengue virus (DENV) free colony was established from eggs collected in Manaus and maintained until the third–fourth generation in order to conduct ZIKV vertical transmission (VT) experiments which used an infectious bloodmeal as the route of virus exposure. The eggs from ZIKV-infected females were allowed to hatch. The resulting F1 progeny (larvae, pupae, and adults) were quantitative polymerase chain reaction (qPCR) assayed for ZIKV. The viability of ZIKV vertically transmitted to F1 progeny was evaluated by cultivation in C6/36 cells. The effects of ZIKV on immature development of Ae. aegypti was assessed and compared with noninfected mosquitoes. AmazonianAe. aegypti were highly susceptible to ZIKV infection (96.7%), and viable virus passed to their progeny via VT. Moreover, eggs from the ZIKV-infected mosquitoes had a significantly lower hatch rate and the slowest hatching. In addition, the larval development period was slower when compared to noninfected, control mosquitoes. This is the first study to illustrate VT initiated by oral infection of the parental population by using mosquitoes, which originated from the field and a ZIKV strain that is naturally circulating in-country. Additionally, this study suggests that ZIKV present in the Ae. aegypti can modify the mosquito life cycle. The data reported here suggest that VT of ZIKV to progeny from naturally infected females may have a critical epidemiological role in the dissemination and maintenance of the virus circulating in the vector.
- Subjects :
- Aedes albopictus
Short Communication
030231 tropical medicine
Population
fitness cost
Aedes aegypti
Mosquito Vectors
Dengue virus
medicine.disease_cause
Arbovirus
Virus
Zika virus
03 medical and health sciences
0302 clinical medicine
Aedes
medicine
Animals
education
030304 developmental biology
Ovum
0303 health sciences
education.field_of_study
General Veterinary
biology
fungi
Zika Virus
biology.organism_classification
medicine.disease
Virology
Flavivirus
Infectious Diseases
Insect Science
Larva
Parasitology
vertical transmission
Female
Fitness cost
Brazil
Subjects
Details
- Language :
- English
- ISSN :
- 19382928 and 00222585
- Volume :
- 56
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- Journal of Medical Entomology
- Accession number :
- edsair.doi.dedup.....c8f63c95d93451fd18270b398aecbb40