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Embryotoxic activity and differential binding of plant-derived carbohydrate-recognizing proteins towards the sea urchin embryo cells
- Source :
- International Journal of Biological Macromolecules. 36:90-97
- Publication Year :
- 2005
- Publisher :
- Elsevier BV, 2005.
-
Abstract
- The embryotoxic activity and differential binding of plant-derived carbohydrate-recognizing proteins on sea urchin ( Lytechinus variegatus ) embryo cells was investigated. IC 50 doses for toxicity on larvae development varied from 0.6 up to 96.3 μg ml −1 and these effects were largely reversed by previously heating the proteins. Changes in the glycoconjungate status of the cell surface were assessed by time-course binding of the proteins during embryogenesis according to their carbohydrate-binding specificity. Glucose/mannose binding-proteins bound embryo cells at the same stage of development, at a similar stage to the N -acetylglucosamine/ N -acetylneuraminic acid binding-protein (WGA) and earlier than galactose specific ones. FITC-conjugates of these proteins confirmed the above results and revealed the presence of specific and differential receptors for them. Inhibition assays using inhibitory glycoproteins significantly diminished the labelled patterns of FITC-conjugates. In conclusion, the assayed proteins exhibited embryotoxicity and their binding requirements were useful for following changes in the pattern of cell surface glycoconjugates on embryo cells of sea urchin. This property could be useful in analyzing other cell types.
- Subjects :
- Embryo, Nonmammalian
Time Factors
Glycoconjugate
Carbohydrates
Mannose
Biology
Mannose-Binding Lectin
Biochemistry
Acetylglucosamine
Inhibitory Concentration 50
chemistry.chemical_compound
Structural Biology
Lectins
biology.animal
Animals
Receptor
Molecular Biology
Sea urchin
Glycoproteins
Plant Proteins
Lytechinus variegatus
chemistry.chemical_classification
Embryogenesis
Temperature
Embryo
General Medicine
Plants
biology.organism_classification
Glucose
chemistry
Sea Urchins
Glycoprotein
Glycoconjugates
Fluorescein-5-isothiocyanate
Protein Binding
Subjects
Details
- ISSN :
- 01418130
- Volume :
- 36
- Database :
- OpenAIRE
- Journal :
- International Journal of Biological Macromolecules
- Accession number :
- edsair.doi.dedup.....c8eeda4da07b0155ba62baacd79241a5