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Effects of Nano-Anatase on Ribulose-1, 5-Bisphosphate Carboxylase/Oxygenase mRNA Expression in Spinach
- Source :
- Biological Trace Element Research. 126:280-289
- Publication Year :
- 2008
- Publisher :
- Springer Science and Business Media LLC, 2008.
-
Abstract
- Being a proven photocatalyst, nano-anatase is capable of undergoing electron transfer reactions under light. In previous studies, we had proven that nano-anatase improved photosynthesis and greatly promoted spinach growth. However, the molecular mechanisms of carbon reaction promoted by nano-anatase are still not clearly understood. In the present paper, reverse transcription polymerase chain reaction and Northern blotting were carried out, and the results showed that the expression of ribulose-1, 5-bisphosphate carboxylase/oxygenase (Rubisco) small subunit (rbcS) and Rubisco large subunit (rbcL) messenger RNA (mRNA) in the nano-anatase-treated spinach were obviously promoted, respectively, while the effects of bulk TiO2 were not so significant. Accordingly, the protein expression of Rubisco from the nano-anatase-treated spinach was increased by 40% compared with control. Further analysis indicated that the activity of Rubisco in the nano-anatase-treated spinach was significantly higher than the control, by up to 2.33 times, and bulk TiO2 treatment had no such significant effects. Together, one of the molecular mechanisms of carbon reaction promoted by nano-anatase is that the nano-anatase treatment results in the enhancement of Rubisco mRNA amounts, the protein levels, and activity of Rubisco, thereby leading to the improvement of Rubisco carboxylation and the high rate of photosynthetic carbon reaction.
- Subjects :
- Oxygenase
Transcription, Genetic
Ribulose-Bisphosphate Carboxylase
Endocrinology, Diabetes and Metabolism
Clinical Biochemistry
Photosynthesis
Biochemistry
Inorganic Chemistry
chemistry.chemical_compound
Spinacia oleracea
RNA, Messenger
Titanium
Ribulose 1,5-bisphosphate
biology
Reverse Transcriptase Polymerase Chain Reaction
fungi
Biochemistry (medical)
RuBisCO
technology, industry, and agriculture
food and beverages
General Medicine
Blotting, Northern
biology.organism_classification
Pyruvate carboxylase
Reverse transcription polymerase chain reaction
Carboxylation
chemistry
biology.protein
Spinach
Subjects
Details
- ISSN :
- 15590720 and 01634984
- Volume :
- 126
- Database :
- OpenAIRE
- Journal :
- Biological Trace Element Research
- Accession number :
- edsair.doi.dedup.....c8e3adbd257ec7d1c59c514007bfcef7