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Minimal motif mapping of a known epitope on human zona pellucida protein-4 using a peptide biosynthesis strategy
- Source :
- Journal of reproductive immunology. 81(1)
- Publication Year :
- 2008
-
Abstract
- An important step in the development of a human zona pellucida (huZP) peptide vaccine is to define the minimal amino acid motif for a mapped B cell epitope peptide within huZP4. Identification of this minimal motif is necessary to remove an overlapping T cell epitope that induces a pathogenic T cell response. Here we describe motif (PLTLEL 314–319 ) mapping of an 18mer B cell epitope peptide 308–325 on huZP4 protein (previously known as huZP1/ZPB protein), achieved using a set of 22 biosynthetic 8mer peptides fused with truncated glutathione S-transferase (GST) or truncated streptavidin protein, and detected using rabbit anti-porcine zona pellucida (pZP) IgG. The immunogenicity of the B cell epitope peptide was evaluated in rabbits using expressed B cell epitope peptide fused with truncated streptavidin as the antigen. This construct elicited high titer antibody to the 18mer B cell epitope peptide, with reactivity to native human ZP, the biosynthetic 18mer peptide and the 18mer peptide GST fusion protein.
- Subjects :
- Male
Swine
Recombinant Fusion Proteins
Immunology
Amino Acid Motifs
Molecular Sequence Data
Epitopes, T-Lymphocyte
Peptide
Biology
Protein Engineering
Zona Pellucida Glycoproteins
Epitope
Pregnancy
medicine
Immunology and Allergy
Animals
Humans
Peptide Biosynthesis
Amino Acid Sequence
Zona pellucida
Glutathione Transferase
chemistry.chemical_classification
Membrane Glycoproteins
Linear epitope
Immunogenicity
Egg Proteins
Obstetrics and Gynecology
Fusion protein
Molecular biology
Peptide Fragments
medicine.anatomical_structure
Reproductive Medicine
chemistry
Antibody Formation
Peptide vaccine
Epitopes, B-Lymphocyte
Female
Rabbits
Streptavidin
Epitope Mapping
Subjects
Details
- ISSN :
- 18727603
- Volume :
- 81
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Journal of reproductive immunology
- Accession number :
- edsair.doi.dedup.....c8d5da13dac289c757e7fe0d070ccebe