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Milling solid proteins to enhance activity after melt-encapsulation
- Source :
- International journal of pharmaceutics. 533(1)
- Publication Year :
- 2017
-
Abstract
- Polymeric systems for the immobilization and delivery of proteins have been extensively used for therapeutic and catalytic applications. While most devices have been created via solution based methods, hot melt extrusion (HME) has emerged as an alternative due to the high encapsulation efficiencies and solvent-free nature of the process. HME requires high temperatures and mechanical stresses that can result in protein aggregation and denaturation, but additives and chemical modifications have been explored to mitigate these effects. This study explores the use of solid-state ball milling to decrease protein particle size before encapsulation within poly(lactic-co-glycolic acid) (PLGA) via HME. The impact of milling on particle dispersion, retained enzymatic activity, secondary structure stability, and release was explored for lysozyme, glucose oxidase, and the virus-like particle derived from Qβ to fully understand the impact of milling on protein systems with different sizes and complexities. The results of this study describe the utility of milling to further increase the stability of protein/polymer systems prepared via HME.
- Subjects :
- Materials science
Drug Compounding
Pharmaceutical Science
Nanotechnology
02 engineering and technology
Protein aggregation
030226 pharmacology & pharmacy
Article
Catalysis
03 medical and health sciences
chemistry.chemical_compound
Glucose Oxidase
0302 clinical medicine
Polylactic Acid-Polyglycolic Acid Copolymer
Glucose oxidase
Lactic Acid
Ball mill
chemistry.chemical_classification
biology
technology, industry, and agriculture
Proteins
Polymer
021001 nanoscience & nanotechnology
PLGA
Drug Liberation
chemistry
biology.protein
Microscopy, Electron, Scanning
Extrusion
Muramidase
Particle size
0210 nano-technology
Polyglycolic Acid
Subjects
Details
- ISSN :
- 18733476
- Volume :
- 533
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- International journal of pharmaceutics
- Accession number :
- edsair.doi.dedup.....c8b3061e032ca79a2312f724df5958ff