Back to Search
Start Over
A hammerhead ribozyme substrate and reporter for in vitro kinetoplastid RNA editing
- Source :
- RNA. 8:548-554
- Publication Year :
- 2002
- Publisher :
- Cold Spring Harbor Laboratory, 2002.
-
Abstract
- Current in vitro assays for RNA editing in kinetoplastids directly examine the products generated by incubation of pre-mRNA substrate with guide RNA (gRNA) and mitochondrial (mt) extract. RNA editing substrates that are modeled on hammerhead ribozymes were designed with catalytic cores that contained or lacked additional uridylates (Us). They proved to be sensitive reporters of editing activity when used for in vitro assays. A deletion editing substrate that is based on A6 pre-mRNA had no ribozyme activity, but its incubation with gRNA and mt extract resulted in its deletion editing and production of a catalytically active ribozyme. Hammerhead ribozymes are thus sensitive tools to assay in vitro RNA editing.
- Subjects :
- Hammerhead ribozyme
Base Sequence
biology
Molecular Sequence Data
Trypanosoma brucei brucei
Ribozyme
RNA
biology.organism_classification
Molecular biology
Biochemistry
RNA editing
biology.protein
Animals
RNA, Catalytic
RNA Editing
Mammalian CPEB3 ribozyme
Guide RNA
Uridine Monophosphate
Molecular Biology
Ligase ribozyme
VS ribozyme
Research Article
Subjects
Details
- ISSN :
- 13558382
- Volume :
- 8
- Database :
- OpenAIRE
- Journal :
- RNA
- Accession number :
- edsair.doi.dedup.....c8abb294d85667b1b0a84618bce7b12d