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Effect of nitrogen source on methanol oxidation and genetic diversity of methylotrophic mixed cultures enriched from pulp and paper mill biofilms
- Source :
- Biodegradation. 22:309-320
- Publication Year :
- 2010
- Publisher :
- Springer Science and Business Media LLC, 2010.
-
Abstract
- Methanol-oxidizing bacteria may play an important role in the development and use of biological treatment systems for the removal of methanol from industrial effluents. Optimization of methanol degradation potential in such systems is contingent on availability of nutrients, such as nitrogen, in the most favorable form and concentration. To that end, this study examined the variation in growth, methanol degradation, and bacterial diversity of two mixed methylotrophic cultures that were provided nitrogen either as ammonium or nitrate and in three different concentrations. Methanol-degrading cultures were enriched from biofilms sampled at a pulp and paper mill and grown in liquid batch culture with methanol as the only carbon source and either ammonium or nitrate as the only added nitrogen source. Results indicate that growth and methanol removal of the mixed cultures increase directly with increased nitrogen, added in either form. However, methanol removal and bacterial diversity, as observed by polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) methods, were higher when using nitrate as the nitrogen source for enrichment and growth, rather than ammonium. Based on results described here, nitrate may potentially be a better nitrogen source when enriching or working with mixed methylotrophic cultures, and possibly more effective when used as a nutrient addition to biofilters.
- Subjects :
- Paper
Environmental Engineering
Nitrogen
Industrial Waste
chemistry.chemical_element
Bioengineering
Biology
Polymerase Chain Reaction
Microbiology
chemistry.chemical_compound
Nitrate
Environmental Chemistry
Ammonium
Effluent
Bacteria
Denaturing Gradient Gel Electrophoresis
business.industry
Methanol
Genetic Variation
Paper mill
Biodegradation
biology.organism_classification
Pollution
chemistry
Biofilms
Environmental chemistry
Methylotroph
business
Oxidation-Reduction
Subjects
Details
- ISSN :
- 15729729 and 09239820
- Volume :
- 22
- Database :
- OpenAIRE
- Journal :
- Biodegradation
- Accession number :
- edsair.doi.dedup.....c8a70671d9c844d59e377b4eaf2bc6ba