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Major Latex Protein MdMLP423 Negatively Regulates Defense against Fungal Infections in Apple
- Source :
- International Journal of Molecular Sciences, Vol 21, Iss 5, p 1879 (2020), International Journal of Molecular Sciences, Volume 21, Issue 5
- Publication Year :
- 2020
- Publisher :
- MDPI AG, 2020.
-
Abstract
- Major latex proteins (MLPs) play critical roles in plants defense and stress responses. However, the roles of MLPs from apple (Malus &times<br />domestica) have not been clearly identified. In this study, we focused on the biological role of MdMLP423, which had been previously characterized as a potential pathogenesis-related gene. Phylogenetic analysis and conserved domain analysis indicated that MdMLP423 is a protein with a &lsquo<br />Gly-rich loop&rsquo<br />(GXGGXG) domain belonging to the Bet v_1 subfamily. Gene expression profiles showed that MdMLP423 is mainly expressed in flowers. In addition, the expression of MdMLP423 was significantly inhibited by Botryosphaeria berengeriana f. sp. piricola (BB) and Alternaria alternata apple pathotype (AAAP) infections. Apple calli overexpressing MdMLP423 had lower expression of resistance-related genes, and were more sensitive to infection with BB and AAAP compared with non-transgenic calli. RNA-seq analysis of MdMLP423-overexpressing calli and non-transgenic calli indicated that MdMLP423 regulated the expression of a number of differentially expressed genes (DEGs) and transcription factors, including genes involved in phytohormone signaling pathways, cell wall reinforcement, and genes encoding the defense-related proteins, AP2-EREBP, WRKY, MYB, NAC, Zinc finger protein, and ABI3. Taken together, our results demonstrate that MdMLP423 negatively regulates apple resistance to BB and AAAP infections by inhibiting the expression of defense- and stress-related genes and transcription factors.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Latex
01 natural sciences
lcsh:Chemistry
Gene Expression Regulation, Plant
Gene expression
MYB
Cloning, Molecular
major latex proteins
lcsh:QH301-705.5
Phylogeny
Spectroscopy
Disease Resistance
Plant Proteins
Zinc finger
Alternaria
General Medicine
Computer Science Applications
Organ Specificity
Malus
fungal infections
apple (malus × domestica)
Protein domain
Flowers
Biology
Article
Catalysis
Microbiology
Inorganic Chemistry
03 medical and health sciences
Protein Domains
Physical and Theoretical Chemistry
Molecular Biology
Gene
Transcription factor
Plant Diseases
Sequence Analysis, RNA
Gene Expression Profiling
fungi
Organic Chemistry
biology.organism_classification
defense and stress responses
WRKY protein domain
030104 developmental biology
lcsh:Biology (General)
lcsh:QD1-999
Saccharomycetales
apple (Malus × domestica)
010606 plant biology & botany
Subjects
Details
- ISSN :
- 14220067
- Volume :
- 21
- Database :
- OpenAIRE
- Journal :
- International Journal of Molecular Sciences
- Accession number :
- edsair.doi.dedup.....c8924d12cc62081b87d23f9d80e1d298