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Phytochemical Screening and Protective Effects of Prunus persica Seeds Extract on Carbon Tetrachloride-Induced Hepatic Injury in Rats
- Source :
- Current Pharmaceutical Biotechnology. 23:158-170
- Publication Year :
- 2022
- Publisher :
- Bentham Science Publishers Ltd., 2022.
-
Abstract
- Background and purpose: Carbon tetrachloride (CCl4) is a dynamic environmental toxin released from chemical factories and its concentration in the atmosphere is accelerating at an alarming proportion. The potential presence of CCl4 in the human body causes liver injury via free radical stimulated inflammatory responses. Objectives: In this study, protective effects of hydromethanolic seeds extract of Prunus persica (PPHM) were evaluated for free radical scavenging potential in CCl4 mediated acute liver toxicity in the murine model. Experimental Approach: Followed by acute oral toxicity analysis, liver cells of Sprague-Dawley (SD) rats were treated with CCl4 and subsequently, the chemoprophylactic effect of extract (400 mg/Kg dose) was evaluated using in vivo studies including, silymarin as the positive control. Biochemical parameters, staining (hematoxylin and eosin (H & E) and Masson’s Trichome) and quantitative gene expression analysis via real-time PCR were used to evaluate hepatic damage control. Results: The results illustrated that PPHM extract exhibit strong anti-oxidant activity, comparable to the positive control, gallic acid. Research study results also demonstrated that the extract treatment at 400 mg/Kg concentration is highly effective in protecting liver damage due to CCl4 exposure. Mechanistic investigations indicated that the therapeutic action of PPHM was correlated with the increase in Nrf2, NQO-1 and decrease in collagen III mRNA genes expression compared to CCl4 treated group. Conclusions and Implications: Accordingly, our research study indicated that PPHM alleviated CCl4-mediated oxidative stress through Nrf2/NQO-1 pathway, thereby protecting liver damage against environmental toxins. Our findings provide supportive evidence to suggest PPHM as a novel nontoxic hepatoprotective agent.
- Subjects :
- Antioxidant
medicine.medical_treatment
Phytochemicals
Pharmaceutical Science
CCL4
Pharmacology
medicine.disease_cause
Antioxidants
Rats, Sprague-Dawley
Mice
chemistry.chemical_compound
In vivo
medicine
Animals
Gallic acid
Hepatoprotective Agent
Carbon Tetrachloride
Prunus persica
Liver injury
Plant Extracts
medicine.disease
Rats
Oxidative Stress
Liver
chemistry
Carbon tetrachloride
Chemical and Drug Induced Liver Injury
Oxidative stress
Biotechnology
Subjects
Details
- ISSN :
- 13892010
- Volume :
- 23
- Database :
- OpenAIRE
- Journal :
- Current Pharmaceutical Biotechnology
- Accession number :
- edsair.doi.dedup.....c886dc4bab2e0da8b7384597671328f2