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Nucleosome Assembly by a Complex of CAF-1 and Acetylated Histones H3/H4
- Source :
- Cell. 87:95-104
- Publication Year :
- 1996
- Publisher :
- Elsevier BV, 1996.
-
Abstract
- Chromatin assembly factor 1 (CAF-1) assembles nucleosomes in a replication-dependent manner. The small subunit of CAF-1 (p48) is a member of a highly conserved subfamily of WD-repeat proteins. There are at least two members of this subfamily in both human (p46 and p48) and yeast cells (Hat2p, a subunit of the B-type H4 acetyltransferase, and Msi1p). Human p48 can bind to histone H4 in the absence of CAF-1 p150 and p60. p48, also a known subunit of a histone deacetylase, copurifies with a chromatin assembly complex (CAC), which contains the three subunits of CAF-1 (p150, p60, p48) and H3 and H4, and promotes DNA replication-dependent chromatin assembly. CAC histone H4 exhibits a novel pattern of lysine acetylation that overlaps with, but is distinct from, that reported for newly synthesized H4 isolated from nascent chromatin. Our data suggest that CAC is a key intermediate of the de novo nucleosome assembly pathway and that the p48 subunit participates in other aspects of histone metabolism.
- Subjects :
- Cell Extracts
DNA Replication
Cytoplasm
DNA, Complementary
Saccharomyces cerevisiae Proteins
Nucleosome assembly
Chromosomal Proteins, Non-Histone
Molecular Sequence Data
Biology
General Biochemistry, Genetics and Molecular Biology
Cell Line
Histones
Histone H4
Histone H1
Acetyltransferases
Histone H2A
Humans
Histone code
Amino Acid Sequence
Histone octamer
Chromatin Assembly Factor-1
Cloning, Molecular
Histone Acetyltransferases
Cell Nucleus
Biochemistry, Genetics and Molecular Biology(all)
Lysine
Chromatin Assembly Factor I
Acetylation
Molecular biology
Chromatin
Nucleosomes
Cell biology
DNA-Binding Proteins
Molecular Weight
Transcription Factors
Subjects
Details
- ISSN :
- 00928674
- Volume :
- 87
- Database :
- OpenAIRE
- Journal :
- Cell
- Accession number :
- edsair.doi.dedup.....c884ea1ce2abd8fc5fbafadbcf5b2a4c