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Effect of Erythropoietin on Morphofunctional Properties of Mesenchymal Stem Cells
- Source :
- Bulletin of Experimental Biology and Medicine. 170:164-170
- Publication Year :
- 2020
- Publisher :
- Springer Science and Business Media LLC, 2020.
-
Abstract
- We studied the effect of erythropoietin on the morphofunctional status of bone marrow mesenchymal stem cells in patients with coronary heart disease. It was shown that the duration of cell exposure with erythropoietin had different effects on the expression levels of adhesion molecules, erythropoietin receptors, and co-expression of the erythropoietin receptor and common β-chain of cytokines, apoptosis/necrosis, and the cell cycle. In most cases, erythropoietin increased proliferation, migration, and NO production by "aged" mesenchymal stem cells (passage 8) and passage 4 mesenchymal stem cells grown during the previous 3 passages in the presence of 33.4 U/ml erythropoietin. Erythropoietin increased the expression of the autophagy marker LC3B in mesenchymal stem cells grown in the presence of erythropoietin in the culture medium. Thus, long-term culturing of mesenchymal stem cells in the presence of erythropoietin in the culture medium increased their resistance to adverse microenvironment factors - oxidative stress and hyperglycemia.
- Subjects :
- 0301 basic medicine
Necrosis
Primary Cell Culture
Cell
Bone Marrow Cells
Cell Count
Coronary Disease
Biology
General Biochemistry, Genetics and Molecular Biology
03 medical and health sciences
0302 clinical medicine
Antigens, CD
Cell Movement
hemic and lymphatic diseases
Autophagy
Receptors, Erythropoietin
medicine
Humans
Erythropoietin
Cell Proliferation
Cell adhesion molecule
Cell Cycle
Mesenchymal stem cell
Mesenchymal Stem Cells
General Medicine
Cell cycle
Erythropoietin receptor
Oxidative Stress
030104 developmental biology
medicine.anatomical_structure
Gene Expression Regulation
Apoptosis
Cancer research
medicine.symptom
Microtubule-Associated Proteins
030217 neurology & neurosurgery
Signal Transduction
medicine.drug
Subjects
Details
- ISSN :
- 15738221 and 00074888
- Volume :
- 170
- Database :
- OpenAIRE
- Journal :
- Bulletin of Experimental Biology and Medicine
- Accession number :
- edsair.doi.dedup.....c881555bb63fad48fefe985a5f933e16